用携带碱性磷酸酶结构基因的质粒转化的大肠杆菌K-12 pho组成型突变体对碱性磷酸酶的排泄。
Excretion of alkaline phosphatase by Escherichia coli K-12 pho constitutive mutants transformed with plasmids carrying the alkaline phosphatase structural gene.
作者信息
Lazzaroni J C, Atlan D, Portalier R C
出版信息
J Bacteriol. 1985 Dec;164(3):1376-80. doi: 10.1128/jb.164.3.1376-1380.1985.
Abstract
Escherichia coli alkaline phosphatase constitutive mutants carrying a pst or a phoS mutation and a plasmid-bearing gene phoA+ excreted into the growth medium up to 50% of the total alkaline phosphatase production. This excretion was pH dependent and did not involve drastic modifications of the cell envelope. Alkaline phosphatase accounted for 80% of total released proteins. Amplification of gene phoA+ was a necessary condition for excretion to occur. When the beta-lactamase structural gene bla+ was coamplified with gene phoA+, both enzymes were excreted. pst-transformed excretory strains did not show the pleiotrophic phenotype previously described for lky mutants.
摘要
携带pst或phoS突变的大肠杆菌碱性磷酸酶组成型突变体以及携带phoA⁺基因的质粒，可将高达50%的总碱性磷酸酶产量分泌到生长培养基中。这种分泌依赖于pH值，且不涉及细胞包膜的剧烈修饰。碱性磷酸酶占总释放蛋白的80%。phoA⁺基因的扩增是发生分泌的必要条件。当β-内酰胺酶结构基因bla⁺与phoA⁺基因共扩增时，两种酶都会被分泌。pst转化的分泌菌株没有表现出先前描述的lky突变体的多效性表型。
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