Inouye H, Michaelis S, Wright A, Beckwith J
J Bacteriol. 1981 May;146(2):668-75. doi: 10.1128/jb.146.2.668-675.1981.
The structural gene for alkaline phosphatase (phoA) of Escherichia coli was cloned into the PstI site of pBR322, from a transducing bacteriophage, lambda p(phoA-proC). The restriction map of the plasmid was established. Based upon this information, several phoA deletion plasmids as well as a smaller phoA+ plasmid were constructed. The genetic map and restriction map were correlated by recombination analysis. Cells carrying one of the phoA+ plasmids overproduce alkaline phosphatase 10-fold upon phosphate limitation. However, both regulation and processing of the enzyme were found to be normal.
大肠杆菌碱性磷酸酶（phoA）的结构基因从转导噬菌体λp（phoA - proC）克隆到pBR322的PstI位点。构建了该质粒的限制性图谱。基于此信息，构建了几个phoA缺失质粒以及一个较小的phoA +质粒。通过重组分析将遗传图谱和限制性图谱关联起来。携带其中一个phoA +质粒的细胞在磷酸盐限制时碱性磷酸酶产量会增加10倍。然而，发现该酶的调节和加工都是正常的。
