Bhandari B, Nicholas D J
J Bacteriol. 1985 Dec;164(3):1383-5. doi: 10.1128/jb.164.3.1383-1385.1985.
The components of the proton motive force (delta p), namely the membrane potential and the transmembrane pH gradient, were measured in washed cells of Rhizobium japonicum CC705 grown in cultures (5% O2-95% N2) in the presence of 10 mM KNO3 and in bacteroids from Glycine max. The delta p and its components remained reasonably constant in cells as well as in bacteroids at various stages of growth. The effects of uncouplers and ATPase inhibitors on the delta p and its components were determined in both cultured cells and bacteroids. The data indicated that a respiration-driven H+ translocation is the source of the delta p in both cultured cells and bacteroids.
在10 mM硝酸钾存在的情况下，于培养物（5%氧气 - 95%氮气）中生长的日本根瘤菌CC705的洗涤细胞以及来自大豆的类菌体中，测量了质子动力（Δp）的组成部分，即膜电位和跨膜pH梯度。在细胞以及处于不同生长阶段的类菌体中，Δp及其组成部分保持相当稳定。在培养细胞和类菌体中都测定了解偶联剂和ATP酶抑制剂对Δp及其组成部分的影响。数据表明，呼吸驱动的H⁺转运是培养细胞和类菌体中Δp的来源。
