Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, S.A., Australia.
Planta. 1984 Jan;161(1):81-5. doi: 10.1007/BF00951463.
Nitrate, nitrite and nitrous oxide were denitrified to N2 gas by washed cells ofRhizobium japonicum CC706 as well as by bacteroids prepared from root nodules ofGlycine max (L.) Merr. (CV. Clark 63). Radiolabelled N2 was produced from either K(15)NO3 or Na(15)NO2 by washed cells ofRh. japonicum CC705 grown with either nitrate only (5 mM) or nitrate (5 mM) plus glutamate (10 mM). Nitrogen gas was also produced from N2O. Similar results were obtained with bacteroids ofG. max. The stoichiometry for the utilization of(15)NO 3 (-) or(15)NO 2 (-) and the produciton of(15)N2 was 2:1 and for N2O utilization and N2 production it was 1:1. Some of the(15)N2 gas produced by denitrification of(15)NO 3 (-) in bacteroids was recycled via nitrogenase into cell nitrogen.
硝态氮、亚硝态氮和一氧化二氮可被 Rhizobium japonicum CC706 的洗涤细胞以及从 Glycine max (L.) Merr. (CV. Clark 63)的根瘤中制备的类菌体还原为 N2 气体。放射性标记的 N2 可由仅用硝酸盐（5 mM）或硝酸盐（5 mM）加谷氨酸（10 mM）培养的 Rh. japonicum CC705 的洗涤细胞从 K(15)NO3 或 Na(15)NO2 中产生。N2O 也可产生氮气。G. max 的类菌体也得到了类似的结果。利用(15)NO 3 (-)或(15)NO 2 (-)和产生(15)N2 的最佳化学计量比为 2:1，而利用 N2O 和产生 N2 的最佳化学计量比为 1:1。一些由类菌体还原(15)NO 3 (-)产生的(15)N2 气体通过固氮酶重新循环到细胞氮中。
