Anitha Thirugnanasambandhar Sivasubramanian, Srikanth Krishnagopal, Suganya Subrayan, Muthukumar Subramanian
1 Central Inter-Disciplinary Research Facility, Sri Balaji Vidyapeeth (Deemed University), Mahatma Gandhi Medical College & Research Institute, Puducherry, India.
2 Department of Ophthalmology, Sri Balaji Vidyapeeth (Deemed University), Mahatma Gandhi Medical College & Research Institute, Puducherry, India.
Eur J Ophthalmol. 2019 Mar;29(2):178-182. doi: 10.1177/1120672118785101. Epub 2018 Jul 11.
: To quantify the levels of nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine in cataractous lenses of smokers and smokers who chewed tobacco in comparison with non-smokers and non-smokers who chewed tobacco.
: A total of 80 cataractous lenses from smokers, non-smokers, smokers with tobacco chewing habit, and non-smokers with tobacco chewing habit were collected from the patients who had enrolled in the Department of Ophthalmology, Mahatma Gandhi Medical College & Research Institute, Puducherry.
: Levels of nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine were quantified using commercially available enzyme-linked immunosorbent assay kits.
: The mean concentrations of lens nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine are as follows: (a) smokers-112.01, 59.57, and 88.91 µmol/L; (b) smokers who chewed tobacco-175.15, 93.95, and 128.72 µmol/L; (c) non-smokers-76.15, 40.65, and 70.20 µmol/L; and (d) non-smokers who chewed tobacco-96.56, 52.87, and 83.88 µmol/L, respectively.
: Nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine at high levels are the major causative agents for cataractogenesis. The results of this study suggest that smoking and tobacco chewing habit generate nitrosative stress that could enhance the pathogenesis for early cataractogenesis.
量化吸烟者和咀嚼烟草的吸烟者白内障晶状体中一氧化氮、诱导型一氧化氮合酶和3-硝基酪氨酸的水平，并与不吸烟者和咀嚼烟草的不吸烟者进行比较。
从在本地治里市圣雄甘地医学院及研究所眼科登记的患者中收集了80个来自吸烟者、不吸烟者、有咀嚼烟草习惯的吸烟者和有咀嚼烟草习惯的不吸烟者的白内障晶状体。
使用市售的酶联免疫吸附测定试剂盒对一氧化氮、诱导型一氧化氮合酶和3-硝基酪氨酸的水平进行量化。
晶状体中一氧化氮、诱导型一氧化氮合酶和3-硝基酪氨酸的平均浓度如下：（a）吸烟者——112.01、59.57和88.91微摩尔/升；（b）咀嚼烟草的吸烟者——175.15、93.95和128.72微摩尔/升；（c）不吸烟者——76.15、40.65和70.20微摩尔/升；（d）咀嚼烟草的不吸烟者——分别为96.56、52.87和83.88微摩尔/升。
高水平的一氧化氮、诱导型一氧化氮合酶和3-硝基酪氨酸是白内障形成的主要致病因素。本研究结果表明，吸烟和咀嚼烟草的习惯会产生亚硝化应激，这可能会增强早期白内障形成的发病机制。
