Ghent University, Department of Plant Biotechnology and Bioinformatics, B-9052 Ghent, Belgium.
VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium.
Plant Physiol. 2018 Sep;178(1):217-232. doi: 10.1104/pp.18.00327. Epub 2018 Jul 10.
In Arabidopsis (), reduced expression of the transcriptional regulator () results in propeller-like rosettes with enlarged and dome-shaped leaves. However, the molecular and cellular processes underlying this peculiar phenotype remain elusive. Here, we studied the interaction between PPD2 and NOVEL INTERACTOR OF JAZ (NINJA) and demonstrated that loss-of-function plants produce rosettes with dome-shaped leaves similar to those of mutants but without the increase in size. We showed that mutants have a convex-shaped primary cell cycle arrest front, putatively leading to excessive cell division in the central leaf blade region. Furthermore, and mutants have a similar increase in the expression of (), and ectopic overexpression of phenocopies the and rosette and leaf shape phenotypes without affecting the size. Our results reveal a pivotal contribution of NINJA in leaf development, in addition to its well-studied function in jasmonate signaling, and imply a new function for D3-type cyclins in, at least partially, uncoupling the size and shape phenotypes of leaves.
在拟南芥中，转录调控因子（）的表达减少会导致螺旋桨状的莲座丛，叶片增大呈圆顶状。然而，这种特殊表型背后的分子和细胞过程仍不清楚。在这里，我们研究了 PPD2 和 NOVEL INTERACTOR OF JAZ（NINJA）之间的相互作用，并表明功能丧失的植物产生的莲座丛具有圆顶状叶片，类似于突变体，但叶片大小没有增加。我们表明，突变体具有凸面形的初级细胞周期阻滞前缘，可能导致中央叶片区域过度分裂。此外，和突变体中（）的表达增加相似，并且异位过表达可模拟和突变体的莲座丛和叶片形状表型，而不影响大小。我们的结果揭示了 NINJA 在叶片发育中的关键作用，除了其在茉莉酸信号转导中研究得很好的功能外，还暗示 D3 型细胞周期蛋白在至少部分地解耦叶片的大小和形状表型方面具有新的功能。
