玉米基因组醇溶蛋白序列的一级结构。
Primary structure of a genomic zein sequence of maize.
作者信息
Hu N T, Peifer M A, Heidecker G, Messing J, Rubenstein I
出版信息
EMBO J. 1982;1(11):1337-42. doi: 10.1002/j.1460-2075.1982.tb01319.x.
The nucleotide sequence of a genomic clone (termed Z4 ) of the zein multigene family was compared to the nucleotide sequence of related cDNA clones of zein mRNAs. A tandem duplication of a 96-bp sequence is found in the genomic clone that is not present in the related cDNA clones. When the duplication is disregarded, the nucleotide sequence homology between Z4 and its related cDNAs was approximately 97%. The nucleotide sequence is also compared to other isolated cDNAs. No introns in the coding region of the zein gene are detected. The first nucleotide of a putative TATA box, TATAAATA , was located 88 nucleotides upstream of the first nucleotide of the first ATG codon which initiated the open reading frame. The first nucleotide of a putative CCAAT box, CAAAAT , appeared 45 nucleotides upstream of the first nucleotide of the zein cDNA clones in the 3' non-coding region also appeared in the genomic sequence at the same locations. The amino acid composition of the polypeptide specified by the Z4 nucleotide sequence is similar to the known composition of zein proteins.
将玉米醇溶蛋白多基因家族的一个基因组克隆（称为Z4）的核苷酸序列与玉米醇溶蛋白mRNA的相关cDNA克隆的核苷酸序列进行了比较。在基因组克隆中发现了一个96碱基对序列的串联重复，而相关cDNA克隆中不存在该重复。当忽略该重复时，Z4与其相关cDNA之间的核苷酸序列同源性约为97%。还将该核苷酸序列与其他分离的cDNA进行了比较。未检测到玉米醇溶蛋白基因编码区的内含子。推测的TATA框TATAAATA的第一个核苷酸位于起始开放阅读框的第一个ATG密码子的第一个核苷酸上游88个核苷酸处。推测的CCAAT框CAAAAT的第一个核苷酸出现在3'非编码区玉米醇溶蛋白cDNA克隆第一个核苷酸上游45个核苷酸处，同样也出现在基因组序列的相同位置。由Z4核苷酸序列指定的多肽的氨基酸组成与已知的玉米醇溶蛋白组成相似。
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