Wisconsin National Primate Research Center, University of Wisconsin, 1220 Capitol Ct., Madison, WI, 53715, USA.
Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, 53707, USA.
Cell Mol Life Sci. 2018 Oct;75(19):3507-3520. doi: 10.1007/s00018-018-2871-3. Epub 2018 Jul 10.
Mesenchymoangioblast (MB) is the earliest precursor for endothelial and mesenchymal cells originating from APLNRPDGFRαKDR mesoderm in human pluripotent stem cell cultures. MBs are identified based on their capacity to form FGF2-dependent compact spheroid colonies in a serum-free semisolid medium. MBs colonies are composed of PDGFRβCD271EMCNDLK1CD73 primitive mesenchymal cells which are generated through endothelial/angioblastic intermediates (cores) formed during first 3-4 days of clonogenic cultures. MB-derived primitive mesenchymal cells have potential to differentiate into mesenchymal stromal/stem cells (MSCs), pericytes, and smooth muscle cells. In this review, we summarize the specification and developmental potential of MBs, emphasize features that distinguish MBs from other mesenchymal progenitors described in the literature and discuss the value of these findings for identifying molecular pathways leading to MSC and vasculogenic cell specification, and developing cellular therapies using MB-derived progeny.
间充质-血管母细胞（MB）是源自人多能干细胞培养物中 APLNRPDGFRαKDR 中胚层的内皮和间充质细胞的最早前体细胞。MB 根据其在无血清半固体培养基中形成 FGF2 依赖性紧密球体集落的能力来鉴定。MB 集落由 PDGFRβCD271EMCNDLK1CD73 原始间充质细胞组成，这些细胞是通过在克隆培养的头 3-4 天形成的内皮/成血管细胞中间产物（核心）产生的。MB 衍生的原始间充质细胞具有分化为间充质基质/干细胞（MSCs）、周细胞和平滑肌细胞的潜力。在这篇综述中，我们总结了 MB 的特征和发育潜力，强调了将 MB 与文献中描述的其他间充质祖细胞区分开来的特征，并讨论了这些发现对于鉴定导致 MSC 和血管生成细胞特化的分子途径以及使用 MB 衍生后代开发细胞疗法的价值。
