Lee C M, Park S-H, Nam M J
1 Department of Biological Science, Gachon University, Seongnam, Republic of Korea.
2 Department of Biological and Chemical Engineering, Hongik University, Seoul, Republic of Korea.
Hum Exp Toxicol. 2019 Jan;38(1):136-147. doi: 10.1177/0960327118785235. Epub 2018 Jul 11.
Many cruciferous vegetables, including cabbage, contain indole-3-carbinol (I3C), which is a known anticarcinogen. However, the anticarcinogenic effects of I3C on liver cancer have not been investigated. Therefore, this study was conducted to evaluate the anticarcinogenic effects of I3C in human hepatocellular carcinoma (HCC) SNU449 cells. The results of MTT and WST-1 assays indicated that treatment of SNU449 cells with I3C decreased viability in dose- and time-dependent manners, while colony formation assays indicated that I3C also inhibited proliferation of SNU449 cells. Moreover, fluorescence-activated cell sorter analysis showed that I3C induced apoptosis in SNU449 cells in dose- and time-dependent manners. Furthermore, terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling revealed that I3C induced DNA fragmentation in SNU449 cells in a time-dependent manner, while Western blotting showed that apoptotic proteins such as p53, cleaved PARP, caspase-3, and caspase-7 were activated in SNU449 cells following treatment with I3C. Finally, reactive oxygen species-related protein peroxiredoxin-1 and thioredoxin-1 expression decreased in I3C-treated SNU449 cells. The aim of our study is to investigate the unknown mechanisms responsible for the apoptotic effects of I3C on human HCC SNU449 cells, and the results suggest that I3C may be useful for the prevention and treatment of liver cancer.
许多十字花科蔬菜，包括卷心菜，都含有吲哚 - 3 - 甲醇（I3C），这是一种已知的抗癌物质。然而，I3C对肝癌的抗癌作用尚未得到研究。因此，本研究旨在评估I3C对人肝细胞癌（HCC）SNU449细胞的抗癌作用。MTT和WST - 1检测结果表明，用I3C处理SNU449细胞会以剂量和时间依赖性方式降低细胞活力，而集落形成检测表明I3C也抑制SNU449细胞的增殖。此外，荧光激活细胞分选分析表明，I3C以剂量和时间依赖性方式诱导SNU449细胞凋亡。此外，末端脱氧核苷酸转移酶脱氧尿苷三磷酸缺口末端标记显示，I3C以时间依赖性方式诱导SNU449细胞中的DNA片段化，而蛋白质印迹显示，在用I3C处理后，SNU449细胞中的凋亡蛋白如p53、裂解的PARP、caspase - 3和caspase - 7被激活。最后，在I3C处理的SNU449细胞中，活性氧相关蛋白过氧化物酶1和硫氧还蛋白1的表达降低。我们研究的目的是探讨I3C对人肝癌SNU449细胞凋亡作用的未知机制，结果表明I3C可能对肝癌的预防和治疗有用。
