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链球菌噬菌体c6A的特性分析

Characterization of streptococcal bacteriophage c6A.

作者信息

Powell I B, Davidson B E

出版信息

J Gen Virol. 1985 Dec;66 ( Pt 12):2737-41. doi: 10.1099/0022-1317-66-12-2737.

DOI:10.1099/0022-1317-66-12-2737
PMID:2999317
Abstract

Bacteriophage c6A is a lytic phage that infects strains of Streptococcus lactis. Infection of S. lactis C6 under standard conditions yielded 124 +/- 8 p.f.u. per infected cell after a latent period of 25 min at 30 degrees C. The virion of c6A was shown to contain at least 12 polypeptides and a 21.9 kilobase double-stranded, linear DNA genome with complementary 5'-protruding single-stranded termini. The (G + C) content of this DNA was estimated to be 36.7%. A restriction map was constructed which indicates that a number of restriction endonucleases did not digest the DNA and that others cleaved with a much lower frequency than expected.

摘要

噬菌体c6A是一种可感染乳酸链球菌菌株的裂解性噬菌体。在标准条件下,用c6A感染乳酸链球菌C6,在30℃下经过25分钟的潜伏期后,每个受感染细胞产生124±8个噬菌斑形成单位。已证明c6A病毒粒子含有至少12种多肽以及一个21.9千碱基的双链线性DNA基因组,其5'端具有互补的突出单链末端。该DNA的(G + C)含量估计为36.7%。构建了一个限制性图谱,结果表明一些限制性内切酶不能消化该DNA,而其他酶的切割频率比预期的要低得多。

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