Sugahara Shintaro, Chiyo Akihiro, Fukuoka Koki, Ueda Yuto, Tokunaga Yuki, Nishida Youichirou, Kinoshita Hideki, Matsuda Yasushi, Igoshi Keiji, Ono Masateru, Yasuda Shin
a Graduate School of Bioscience , Tokai University , Kumamoto City , Japan.
b School of Agriculture , Tokai University , Kumamoto City , Japan.
Biosci Biotechnol Biochem. 2018 Nov;82(11):1973-1984. doi: 10.1080/09168451.2018.1495552. Epub 2018 Jul 11.
This study aimed to investigate the unique antioxidative effects of Japanese moringa products, herbal leaf tea and stem tea, using established free radical assays, focusing on superoxide anion (O) radical generation systems. Hot-water extracts from moringa teas resulted in different but lower scavenging activities than Trolox in four synthetic free radical models. Interestingly, these extracts further showed higher O radical scavenging effects than Trolox in the phenazine methosulfate-NADH-nitroblue tetrazolium and xanthine oxidase assay systems. Incubating human neutrophils in the presence of these tea extracts rather than Trolox effectively suppressed cellular O radical generation. Among the eight known phenolic constituents of moringa leaves, caffeic acid and chlorogenic acid may be responsible for the Ospecific radical scavenging capacity stronger than that of Trolox. These results suggest that moringa herbal teas are a good source of natural antioxidants for preventing O radical-mediated disorders. Abbreviations: O: superoxide anion; ROS: reactive oxygen species; HO: hydrogen peroxide; XOD: xanthine oxidase; DPPH: 1,1-diphenyl-2-picrylhydrazyl; ABTS: 2,2'-azinobis(2-ethylbenzothiazoline-6-sulfonic acid) cation; CPZ: chlorpromazine cation; PMS: phenazine methosulfate; NBT: nitroblue tetrazolium; PMA: phorbol 12-myristate 13-acetate.
本研究旨在利用已建立的自由基检测方法，研究日本辣木产品（叶茶和茎茶）独特的抗氧化作用，重点关注超氧阴离子（O）自由基生成系统。在四种合成自由基模型中，辣木叶茶的热水提取物产生了不同但低于Trolox的清除活性。有趣的是，在硫酸吩嗪 - 烟酰胺腺嘌呤二核苷酸 - 硝基蓝四唑和黄嘌呤氧化酶检测系统中，这些提取物对O自由基的清除效果比Trolox更高。在这些茶提取物而非Trolox存在的情况下孵育人中性粒细胞，可有效抑制细胞O自由基的产生。在辣木叶的八种已知酚类成分中，咖啡酸和绿原酸可能是其对O自由基清除能力比Trolox更强的原因。这些结果表明，辣木叶茶是预防O自由基介导疾病的天然抗氧化剂的良好来源。缩写：O：超氧阴离子；ROS：活性氧；HO：过氧化氢；XOD：黄嘌呤氧化酶；DPPH：1,1 - 二苯基 - 2 - 苦基肼；ABTS：2,2'- 偶氮二（2 - 乙基苯并噻唑啉 - 6 - 磺酸）阳离子；CPZ：氯丙嗪阳离子；PMS：硫酸吩嗪；NBT：硝基蓝四唑；PMA：佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯。
