Zhuang Xiao-Cui, Chen Gui-Lin, Liu Ye, Zhang Yong-Li, Guo Ming-Quan
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Antioxidants (Basel). 2021 Mar 1;10(3):370. doi: 10.3390/antiox10030370.
, also known as "green heart," is widely used for the treatment of various diseases as a traditional ethnomedicinal plant in local communities in Africa. In this work, 9 and 12 potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands from were quickly screened out by combining SOD and XOD affinity ultrafiltration with LC-MS, respectively. In this way, four new lignanamides (compounds -) and one new macrocyclic glycoside (compound ), along with three known compounds (compounds , , and ), were isolated and identified firstly in this species. The structures of the new compounds were elucidated by spectroscopic analysis, including NMR and UPLC-QTOF-MS/MS. Among these compounds, compound showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities, and total ferric-reducing antioxidant power (FRAP) with IC values of 6.405 ± 0.362 µM, 5.381 ± 0.092 µM, and 17.488 ± 1.625 mmol TE/g, respectively. Moreover, compound displayed the highest inhibitory activity on cyclooxygenase-2 (COX-2) with IC value of 0.123 ± 0.004 µM, and the ranking order of other compounds' IC values was > > > > . The present study suggested that lignanamides might represent interesting new characteristic functional components of to exert remarkable antioxidant and anti-inflammatory activities. Moreover, compound , a new arylnaphthalene lignanamide, would be a highly potential natural antioxidant and anti-inflammatory agent from .
（该植物）也被称为“绿心”，作为一种传统民族药用植物，在非洲当地社区被广泛用于治疗各种疾病。在这项研究中，通过将超氧化物歧化酶（SOD）和黄嘌呤氧化酶（XOD）亲和超滤与液相色谱 - 质谱联用，分别从（该植物）中快速筛选出9种和12种潜在的SOD和XOD配体。通过这种方式，首次从该物种中分离并鉴定出四种新的木脂酰胺（化合物 - ）和一种新的大环糖苷（化合物 ），以及三种已知化合物（化合物 、 和 ）。新化合物的结构通过光谱分析得以阐明，包括核磁共振（NMR）和超高效液相色谱 - 四极杆飞行时间质谱联用（UPLC - QTOF - MS/MS）。在这些化合物中，化合物 表现出最高的1,1 - 二苯基 - 2 - 苦基肼（DPPH）、2,2'- 偶氮二（3 - 乙基苯并噻唑啉）- 6 - 磺酸（ABTS）自由基清除活性以及总铁还原抗氧化能力（FRAP），其半数抑制浓度（IC）值分别为6.405±0.362 μM、5.381±0.092 μM和17.488±1.625 mmol TE/g。此外，化合物 对环氧合酶 - 2（COX - 2）表现出最高的抑制活性，IC值为0.123±0.004 μM，其他化合物IC值的排序为 ＞ ＞ ＞ ＞ 。本研究表明，木脂酰胺可能是（该植物）中具有显著抗氧化和抗炎活性的有趣新特征功能成分。此外，化合物 ，一种新的芳基萘木脂酰胺，将是来自（该植物）的极具潜力的天然抗氧化剂和抗炎剂。
