New Lignanamides with Antioxidant and Anti-Inflammatory Activities Screened Out and Identified from Combining Affinity Ultrafiltration LC-MS with SOD and XOD Enzymes.

, also known as "green heart," is widely used for the treatment of various diseases as a traditional ethnomedicinal plant in local communities in Africa. In this work, 9 and 12 potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands from were quickly screened out by combining SOD and XOD affinity ultrafiltration with LC-MS, respectively. In this way, four new lignanamides (compounds -) and one new macrocyclic glycoside (compound ), along with three known compounds (compounds , , and ), were isolated and identified firstly in this species. The structures of the new compounds were elucidated by spectroscopic analysis, including NMR and UPLC-QTOF-MS/MS. Among these compounds, compound showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities, and total ferric-reducing antioxidant power (FRAP) with IC values of 6.405 ± 0.362 µM, 5.381 ± 0.092 µM, and 17.488 ± 1.625 mmol TE/g, respectively. Moreover, compound displayed the highest inhibitory activity on cyclooxygenase-2 (COX-2) with IC value of 0.123 ± 0.004 µM, and the ranking order of other compounds' IC values was > > > > . The present study suggested that lignanamides might represent interesting new characteristic functional components of to exert remarkable antioxidant and anti-inflammatory activities. Moreover, compound , a new arylnaphthalene lignanamide, would be a highly potential natural antioxidant and anti-inflammatory agent from .