IEEE Trans Nanobioscience. 2018 Oct;17(4):402-408. doi: 10.1109/TNB.2018.2853269. Epub 2018 Jul 5.
Bioimpedance spectroscopy is a promising method for tissue ischemia monitoring; it is used in this research to study ischemia and cell morphology using bioimpedance measurements of rabbit liver tissue. This paper presents the evolution of Cole parameters and cell parameters with time and establishes a relation between cellular morphological changes during cell ischemia with bioimpedance measurements at different frequencies. The general behavior of ischemic liver tissue has been analyzed. Evolution of Cole parameters was extrapolated from BIS measurements, and their behavior was studied with respect to time. Then, cell parameters were estimated from Cole parameters. A detailed analysis of the changes of cell parameters was done in parallel with explanations for these changes depending on the physiological mechanism of ischemia and necrosis bioprocess. These explanations give a confirmation of the established relation between cell morphological changes and the corresponding BIS measurements.
生物阻抗谱是一种很有前途的组织缺血监测方法；本研究使用它通过对兔肝组织的生物阻抗测量来研究缺血和细胞形态。本文介绍了随时间推移的Cole 参数和细胞参数的演变，并建立了细胞缺血过程中细胞形态变化与不同频率下生物阻抗测量之间的关系。分析了缺血肝组织的一般行为。从 BIS 测量中推断出 Cole 参数的演变，并研究了它们随时间的变化。然后，从 Cole 参数中估计出细胞参数。与根据缺血和坏死生物过程的生理机制对这些变化的解释并行，对细胞参数的变化进行了详细分析。这些解释证实了细胞形态变化与相应的 BIS 测量之间建立的关系。
