Eren Sevki Hakan, Korkmaz Ilhan, Guven Fatma Mutlu Kukul, Tekin Yusuf Kenan, Ozdemir Levent
Department of Emergency Medicine, Medicine Faculty, Gaziantep University, Gaziantep, Turkey.
Department of Emergency Medicine, Medicine Faculty, Cumhuriyet University, Sivas, Turkey.
Clinics (Sao Paulo). 2018 Jul 10;73:e16550. doi: 10.6061/clinics/2018/e16-550.
Consumption of toxic species of mushrooms may have detrimental effects and increase oxidative stress. Paraoxonase, arylesterase and glutathione-S-transferase are antioxidants that resist oxidative stress. In this study, we analyzed the changes in these enzymes during intoxication due to mushrooms.
The study enrolled 49 adult patients with a diagnosis of mushroom poisoning according to clinical findings and 49 healthy volunteers as the control group. The patients with mild clinical findings were hospitalized due to the possibility that the patient had also eaten the mushrooms and due to clinical findings in the late period, which could be fatal. Paraoxonase, arylesterase, and glutathione-S-transferase concentrations, as well as total antioxidant and oxidant status, were determined in the 49 patients and 49 healthy volunteers by taking blood samples in the emergency department.
While paraoxonase, arylesterase, and total antioxidant status were significantly decreased in the patient group (p<0.05), glutathione-S-transferase, total oxidant status and the oxidative stress index were significantly higher (p<0.05). There was a positive correlation between the hospitalization time and the oxidative stress index (r=0.752, p<0.001), whereas a negative correlation was found with glutathione-S-transferase (r=-0.420, p=0.003).
We observed a significant decrease in paraoxonase and arylesterase and an increase in glutathione-S-transferase and oxidative stress indexes in patients with mushroom poisoning, indicating that these patients had an oxidative status. In particular, a low total antioxidant status and high oxidative stress index may gain importance in terms of the assessment of hospitalization duration.
食用有毒蘑菇可能会产生有害影响并增加氧化应激。对氧磷酶、芳基酯酶和谷胱甘肽 - S - 转移酶是抵抗氧化应激的抗氧化剂。在本研究中，我们分析了蘑菇中毒期间这些酶的变化。
该研究纳入了49例根据临床症状诊断为蘑菇中毒的成年患者，并以49名健康志愿者作为对照组。临床症状较轻的患者因有可能食用了蘑菇以及后期可能致命的临床症状而住院。在急诊科采集49例患者和49名健康志愿者的血样，测定对氧磷酶、芳基酯酶和谷胱甘肽 - S - 转移酶的浓度，以及总抗氧化和氧化状态。
患者组中对氧磷酶、芳基酯酶和总抗氧化状态显著降低（p<0.05），而谷胱甘肽 - S - 转移酶、总氧化状态和氧化应激指数显著升高（p<0.05）。住院时间与氧化应激指数呈正相关（r = 0.752，p<0.001），而与谷胱甘肽 - S - 转移酶呈负相关（r = -0.420，p = 0.003）。
我们观察到蘑菇中毒患者的对氧磷酶和芳基酯酶显著降低，谷胱甘肽 - S - 转移酶和氧化应激指数升高，表明这些患者存在氧化状态。特别是，低总抗氧化状态和高氧化应激指数在评估住院时间方面可能具有重要意义。
