Alizadeh Mehdi, Nasiri Mahboobeh, Samadi Morteza, Ghasemi Nasrin, Moradi Ali
International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.
Int J Reprod Biomed. 2021 Jul 27;19(6):559-568. doi: 10.18502/ijrm.v19i6.9377. eCollection 2021 Jun.
Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL.
To investigate the relationship between Q192R and L55M polymorphisms of as antioxidant enzyme and the risk of RPL.
In this case-control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method.
Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39-86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic frequency in the RPL participants compared to the control group (p = 0.53). Also, statistical analysis of the L55M polymorphism for MM genotype in the case group compared with the control group showed a significant difference (OR = 3.59, CI = 0.97-13.30, p = 0.042), but none for the LM and LL genotypes.
The findings showed a significant correlation between the Q192R polymorphisms and the L55M enzyme and RPL in this study population.
复发性流产（RPL）是指妊娠前半期发生两次或两次以上流产的情况。氧化应激被认为在复发性流产中起核心作用。
研究抗氧化酶的Q192R和L55M多态性与复发性流产风险之间的关系。
在这项病例对照研究中，纳入了110名复发性流产女性（病例组）和110名健康可育女性（对照组），她们均来自伊朗设拉子不孕不育研究与临床中心。从所有参与者的外周血中提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性方法对多态性进行基因分型。
Q192R多态性的统计分析显示，病例组和对照组之间RR基因型存在显著差异（OR = 11，CI = 1.39 - 86.87，p = 0.005），但QR和QQ基因型无差异。与对照组相比，复发性流产参与者中R和Q等位基因频率未观察到显著关联（p = 0.53）。此外，病例组与对照组相比，L55M多态性的MM基因型统计分析显示存在显著差异（OR = 3.59，CI = 0.97 - 13.30，p = 0.042），但LM和LL基因型无差异。
本研究结果表明，该研究人群中Q192R多态性和L55M酶与复发性流产之间存在显著相关性。
