成功和失败的微型种植体：微生物学评估和细菌内毒素定量分析。
Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin.
机构信息
Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, São Paulo, Brasil.
Universidade de Guarulhos, Divisão de Pesquisa Odontológica, Departamento de Periodontia, Guarulhos, São Paulo, Brasil.
出版信息
J Appl Oral Sci. 2018 Jul 10;26:e20170631. doi: 10.1590/1678-7757-2017-0631.
OBJECTIVES
Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay.
MATERIAL AND METHODS
The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05).
RESULTS
All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p>0.05).
CONCLUSION
Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants.
目的
本体内研究使用两组微型种植体（成功和失败），目的是：通过棋盘式 DNA-DNA 杂交技术评估微生物污染，并通过鲎变形细胞溶解物测定法定量细菌内毒素。
材料与方法
从 15 名接受正畸治疗的患者中获得了 15 个成功和 10 个失败的微型种植体（直径 1.6 毫米×长 7.0 或 9.0 毫米），分别置于上颌骨和/或下颌骨。使用 SAS 软件（α=0.05）对 Wilcoxon 秩和检验进行统计数据分析。
结果
两组微型种植体均检测到所有 40 种微生物，且存在不同的频率。两组间在微生物群落（蓝色、紫色、黄色、绿色、橙色、红色和其他物种）和内毒素定量方面无差异（p＞0.05）。
结论
微型种植体早期稳定性丧失既不是微生物污染也不是内毒素定量的决定因素。
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