Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Uppsala, Sweden.
Helmholtz Institute for RNA-based Infection Research (HIRI), Würzburg, Germany.
Nat Rev Microbiol. 2018 Oct;16(10):601-615. doi: 10.1038/s41579-018-0049-5.
RNA-binding proteins (RBPs) are central to most if not all cellular processes, dictating the fate of virtually all RNA molecules in the cell. Starting with pioneering work on ribosomal proteins, studies of bacterial RBPs have paved the way for molecular studies of RNA-protein interactions. Work over the years has identified major RBPs that act on cellular transcripts at the various stages of bacterial gene expression and that enable their integration into post-transcriptional networks that also comprise small non-coding RNAs. Bacterial RBP research has now entered a new era in which RNA sequencing-based methods permit mapping of RBP activity in a truly global manner in vivo. Moreover, the soaring interest in understudied members of host-associated microbiota and environmental communities is likely to unveil new RBPs and to greatly expand our knowledge of RNA-protein interactions in bacteria.
RNA 结合蛋白 (RBPs) 是大多数（如果不是全部）细胞过程的核心，决定了细胞中几乎所有 RNA 分子的命运。从核糖体蛋白的开创性工作开始，对细菌 RBPs 的研究为 RNA-蛋白质相互作用的分子研究铺平了道路。多年来的工作已经确定了主要的 RBPs，它们在细菌基因表达的各个阶段作用于细胞转录本，并使它们能够整合到包含小非编码 RNA 的转录后网络中。细菌 RBP 研究现在已经进入了一个新时代，基于 RNA 测序的方法可以在体内以真正全面的方式绘制 RBP 活性图谱。此外，人们对宿主相关微生物组和环境群落中研究不足的成员的兴趣日益浓厚，这很可能会揭示新的 RBPs，并极大地扩展我们对细菌中 RNA-蛋白质相互作用的认识。
