Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892-5430, USA.
Curr Genet. 2020 Aug;66(4):713-717. doi: 10.1007/s00294-020-01066-y. Epub 2020 Mar 19.
It is now established that base-pairing regulatory RNAs are key players in post-transcriptional regulatory networks where they affect the translation and/or stability of their target RNAs. In many cases, the base-pairing between two RNAs is facilitated by an RNA-binding protein (RBP) that serves as an RNA chaperone. Recent advances in sequencing methods have revealed the RNA populations bound by the RBPs, yielding insights valuable into regulatory networks. Further analyses of these networks can improve our understanding of the roles played by RBPs in the regulation of gene expression by regulatory RNAs, especially when multiple RBPs are involved. For example, using an RNA sequencing-based methodology that captures RNA-RNA pairs on RBP, an interplay between two RBPs in bacteria that compete on the same RNA-RNA pair was revealed. In this case, one protein promotes negative regulation of the target RNA, while the second protein can block this regulation. In this mini-review, I outline the exciting future directions that can be taken to deepen our understanding of the roles played by RBPs in post-transcriptional regulation, and discuss how the different sequencing methods can assist in deciphering the relationships among RBPs, and between the RBPs and the RNAs they bind. Having a more detailed picture of the RBPs-RNAs network will elucidate how bacteria can have nuanced control of gene expression, critical for survival in the varied environments in which bacteria live.
现在已经确定，碱基配对调节 RNA 是转录后调节网络中的关键参与者，它们影响其靶 RNA 的翻译和/或稳定性。在许多情况下，两个 RNA 之间的碱基配对是由充当 RNA 伴侣的 RNA 结合蛋白 (RBP) 促进的。测序方法的最新进展揭示了 RBPs 结合的 RNA 群体，为调节网络提供了有价值的见解。对这些网络的进一步分析可以提高我们对 RBP 在调节 RNA 对基因表达的作用的理解，特别是当涉及多个 RBP 时。例如，使用一种基于 RNA 测序的方法，该方法可以捕获 RBP 上的 RNA-RNA 对，揭示了细菌中两个 RBP 之间的相互作用，它们在同一 RNA-RNA 对上竞争。在这种情况下，一种蛋白质促进靶 RNA 的负调节，而第二种蛋白质可以阻止这种调节。在这篇综述中，我概述了可以深入了解 RBP 在转录后调节中所扮演的角色的令人兴奋的未来方向，并讨论了不同的测序方法如何有助于破译 RBPs 之间以及它们与结合的 RNA 之间的关系。更详细地了解 RBPs-RNAs 网络将阐明细菌如何对基因表达进行细微的控制，这对于细菌在其生存的各种环境中生存至关重要。
