Ali Islam A Abdelaziz, Neelakantan Prasanna
Discipline of Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Dent J (Basel). 2018 Jul 10;6(3):31. doi: 10.3390/dj6030031.
Light activated disinfection (LAD) is a strategy for optimizing root canal disinfection by using a highly-selective, targeted killing of bacteria using a combination of photosensitizers and light. Over the past decade, numerous in vitro and clinical studies have been performed to demonstrate the effectiveness of this mode of root canal disinfection. While most studies offer an important understanding of the effectiveness of LAD on monospecies biofilms, few have offered credence to the fact that infections of the root canal system are mediated by polymicrobial biofilms. Hence, it is imperative to understand the effect of LAD on polymicrobial biofilms both in terms of microbial killing and the changes in the biofilm architecture. The aim of this review was to systematically review the literature to evaluate the effect of LAD on dual and multispecies biofilms and demonstrate the antibiofilm effect of LAD. Two databases (PubMed and Scopus) were searched to identify eligible studies using a combination of key words. These studies were reviewed to draw conclusions on the effect of LAD on dual and multi species biofilm and the antibiofilm effect of LAD. It was found that LAD alone may be unable to eradicate dual and multispecies biofilms, but it may enhance the effect of conventional canal debridement strategies. Novel formulations of photosensitizers with nanoparticles showed the potential to inhibit biofilm formation and/or disrupt the biofilm architecture.
光活化消毒（LAD）是一种通过联合使用光敏剂和光对细菌进行高度选择性、靶向杀伤来优化根管消毒的策略。在过去十年中，已经进行了大量的体外和临床研究来证明这种根管消毒方式的有效性。虽然大多数研究对LAD对单一菌种生物膜的有效性提供了重要的认识，但很少有研究证实根管系统感染是由多菌种生物膜介导的这一事实。因此，有必要从微生物杀灭和生物膜结构变化两方面了解LAD对多菌种生物膜的影响。本综述的目的是系统回顾文献，以评估LAD对双菌种和多菌种生物膜的影响，并证明LAD的抗生物膜作用。通过搜索两个数据库（PubMed和Scopus），使用关键词组合来识别符合条件的研究。对这些研究进行综述，以得出关于LAD对双菌种和多菌种生物膜的影响以及LAD的抗生物膜作用的结论。研究发现，单独使用LAD可能无法根除双菌种和多菌种生物膜，但它可能增强传统根管清创策略的效果。含有纳米颗粒的新型光敏剂配方显示出抑制生物膜形成和/或破坏生物膜结构的潜力。
