Ghadirian E, Kongshavn P A
Parasite Immunol. 1985 Sep;7(5):479-87. doi: 10.1111/j.1365-3024.1985.tb00093.x.
The role of the spleen in amoebic infection was examined in mice, using strains selected as being either genetically-susceptible (C57BL/6) or genetically-resistant (A/J) to amoebiasis. Splenectomized and sham-operated animals were inoculated intracaecally with 2.5 X 10(5) polyxenic trophozoites of E. histolytica at 6, 12 and 15 days post-splenectomy. The animals were killed 6 or 12 days after infection and the parasite burden was evaluated. Removal of the spleen in both susceptible and resistant mouse strains rendered these hosts extremely resistant to amoebic infection by this criterion. Gross examination of the caeca of non-splenectomized, genetically-susceptible mice showed numerous ulcers over the mucosal surface when compared to the splenectomized group which had superficial lesions or none. These observations suggest that the spleen plays a suppressive role in early anti-amoebic resistance.
利用对阿米巴病具有遗传易感性（C57BL/6）或遗传抗性（A/J）的品系小鼠，研究脾脏在阿米巴感染中的作用。在脾切除术后第6、12和15天，对脾切除和假手术的动物经盲肠内接种2.5×10⁵个溶组织内阿米巴多菌型滋养体。在感染后6天或12天处死动物，并评估寄生虫负荷。按照这一标准，切除易感和抗性小鼠品系的脾脏均使这些宿主对阿米巴感染具有极强的抗性。与有浅表病变或无病变的脾切除组相比，对未进行脾切除、具有遗传易感性的小鼠的盲肠进行大体检查发现，其粘膜表面有大量溃疡。这些观察结果表明，脾脏在早期抗阿米巴抗性中起抑制作用。
