Goudarzi Mansour, Mobarez Ashraf Mohabati, Najar-Peerayeh Shahin, Mirzaee Mohsen
Department of Medical Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Iran J Microbiol. 2018 Apr;10(2):74-81.
The antibiotic resistance among strains has increased worldwide. Additionally, biofilm-forming isolates of play an important role in human infections. This study was conducted to investigate the prevalence of virulence and antibiotic resistance genes between biofilm-producing and non-biofilm-producing strains.
In this study, 228 isolates from clinical and environmental specimens were obtained from different wards of hospitals in Lorestan province (Iran). Then, the pattern of antibiotic resistance and minimum inhibitory concentration (MIC) against β-lactams, glycopeptides, aminoglycosides and other common antibiotics was investigated using disk diffusion and agar dilution methods. Biofilm formation was investigated using polystyrene microtiter plates. PCR assay was conducted for antibiotic resistance and biofilm related genes. Pulse field gel electrophoresis (PFGE) was used to determine the clonal spread of isolates.
Most of isolates (78%) were resistant to penicillin, but all were susceptible to linezolid and tigecycline. The biofilm-producing isolates were more resistant to β-lactams, glycopeptides and aminoglycosides compared to non-biofilm-producing strains. In biofilm-producing isolates, and were the dominant virulence genes and and genes were the dominant resistant genes. PFGE analysis exhibited a similar pattern between the clinical and environmental isolates, suggesting the presence of a common origin of the infection by .
The results of the antibiotic resistance, biofilm assay, and PFGE analysis suggest that there is a common clone of persistent and biofilm-producing strains of , which could rapidly disseminate in patients and the environment.
全球范围内菌株的抗生素耐药性有所增加。此外，具有生物膜形成能力的分离株在人类感染中起重要作用。本研究旨在调查产生物膜和不产生物膜的菌株之间毒力基因和抗生素耐药基因的流行情况。
在本研究中，从伊朗洛雷斯坦省医院的不同病房获取了228株来自临床和环境标本的分离株。然后，采用纸片扩散法和琼脂稀释法研究了对β-内酰胺类、糖肽类、氨基糖苷类及其他常用抗生素的耐药模式和最低抑菌浓度（MIC）。使用聚苯乙烯微量滴定板研究生物膜形成情况。对抗生素耐药基因和生物膜相关基因进行PCR检测。采用脉冲场凝胶电泳（PFGE）来确定分离株的克隆传播情况。
大多数分离株（78%）对青霉素耐药，但对利奈唑胺和替加环素均敏感。与不产生物膜的菌株相比，产生物膜的分离株对β-内酰胺类、糖肽类和氨基糖苷类更具耐药性。在产生物膜的分离株中，[具体基因1]和[具体基因2]是主要的毒力基因，[具体基因3]和[具体基因4]是主要的耐药基因。PFGE分析显示临床分离株和环境分离株之间存在相似模式，表明[菌株名称]感染存在共同来源。
抗生素耐药性、生物膜检测和PFGE分析结果表明，存在[菌株名称]的持续存在且产生物膜菌株的共同克隆，其可在患者和环境中迅速传播。
