Li Qiang, Fu Yu, Sun Qun, Sourzat Pierre, Yang Mei, Liu Chengyi, Tan Hao, Ye Lei, Zou Jie, Wu Chenguang, Zhang Bo, Li Xiaolin
Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu, Sichuan, China.
Mycobiology. 2018 Mar 29;46(1):64-71. doi: 10.1080/12298093.2018.1454005. eCollection 2018.
The aim of this study was to investigate the pattern of distribution of mating type () genes of in ectomycorhizosphere soils from natural -producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex PCR were used to weight the copy numbers of and in natural truffle soils and cultivated orchard soils. The effect of limestone on the pattern of truffle genes and the correlation between soil properties and the proportion of genes were also assessed. These results indicated that an uneven and nonrandom distribution of genes was common in truffle-producing areas, cultivated truffle orchards, and ascocarps gleba. The competition between the two mating type genes and the expansion of unbalanced distribution was found to be closely related to truffle fructification. Limestone treatments failed to alter the proportion of the two mating type genes in the soil. The content of available phosphorus in soil was significantly correlated with the value of / in cultivated and natural ectomycorhizosphere soils. The application of real-time quantitative PCR can provide reference for monitoring the dynamic changes of mating type genes in soil. This study investigates the distributional pattern of genes in the ectomycorhizosphere soil and ascocarp gleba from different regions, which may provide a foundation for the cultivation of .
本研究的目的是调查来自天然松露产区和人工栽培松露果园的外生菌根际土壤以及不同地区子囊果样本中松露（）交配型基因的分布模式。采用定量实时PCR和多重PCR技术来衡量天然松露土壤和人工栽培果园土壤中及的拷贝数。还评估了石灰石对松露基因模式的影响以及土壤性质与基因比例之间的相关性。这些结果表明，在松露产区、人工栽培松露果园和子囊果菌髓中，基因的不均匀和非随机分布很常见。发现两种交配型基因之间的竞争以及不平衡分布的扩大与松露结实密切相关。石灰石处理未能改变土壤中两种交配型基因的比例。在人工栽培和天然外生菌根际土壤中，土壤有效磷含量与/值显著相关。实时定量PCR的应用可为监测土壤中交配型基因的动态变化提供参考。本研究调查了不同地区外生菌根际土壤和子囊果菌髓中基因的分布模式，这可能为松露的栽培提供基础。
