Department of Ophthalmology, Peking University Third Hospital; Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing 100191, China.
Chin Med J (Engl). 2018 Jul 20;131(14):1710-1714. doi: 10.4103/0366-6999.235883.
Endothelium allotransplantation is the primary treatment for corneal decompensation. The worldwide shortage of donor corneal tissue has led to increasing pressure to seek an alternative for surgical restoration of corneal endothelium. Compressed collagen (CC) gels have excellent biocompatibility, simple preparation course and easy to be manipulated. This study aimed to form a new biomimetic endothelium graft by CC.
We expanded bovine corneal endothelial cells (B-CECs) on laminin-coated CC to form a biomimetic endothelium graft. Scanning electron microscope was used for ultrastructural analysis and tight junction protein ZO-1 expression was tested by immunohistochemistry.
The biomimetic endothelium graft, we conducted had normal cell morphology, ultrastructure and higher cell density (3612.2 ± 43.4 cells/mm). ZO-1 localization at B-CECs membrane indicated the bioengineered graft possess the basic endothelium function.
A.
biomimetic endothelium graft with B-CECs expanded on CC sheet was constructed, which possessed cells' morphology similar to that of in vivo endothelial cells and specific basic function of endothelium layer. This method provided the possibility of using one donor's cornea to form multiple uniformed endothelium grafts so as to overcome the shortage of cadaveric cornea tissue.
同种异体角膜内皮移植是角膜失代偿的主要治疗方法。全球供体角膜组织短缺，导致对外科恢复角膜内皮的替代方法的需求不断增加。压缩胶原 (CC) 凝胶具有极好的生物相容性、简单的制备过程和易于操作的特点。本研究旨在通过 CC 形成一种新的仿生内皮移植物。
我们在层粘连蛋白包被的 CC 上扩展牛角膜内皮细胞 (B-CEC) 以形成仿生内皮移植物。扫描电子显微镜用于超微结构分析，免疫组织化学检测紧密连接蛋白 ZO-1 的表达。
我们构建的仿生内皮移植物具有正常的细胞形态、超微结构和更高的细胞密度（3612.2±43.4 个/平方毫米）。B-CEC 细胞膜上 ZO-1 的定位表明生物工程移植物具有基本的内皮功能。
A. 在 CC 片上扩展的 B-CEC 构建了仿生内皮移植物，其细胞形态类似于体内内皮细胞，具有特定的内皮层基本功能。该方法提供了使用一个供体角膜形成多个均匀的内皮移植物的可能性，从而克服了尸体角膜组织短缺的问题。
