噬菌体T4 uvsY基因的核苷酸序列。

Nucleotide sequence of bacteriophage T4 uvsY gene.

作者信息

Takahashi H, Kobayashi M, Noguchi T, Saito H

出版信息

Virology. 1985 Dec;147(2):349-53. doi: 10.1016/0042-6822(85)90137-0.

Abstract

We have determined the nucleotide sequence of a 1001-bp region comprising the uvsY gene of bacteriophage T4. An open reading frame of 420 base pairs was found to encode the uvsY gene product. The uvsY gene comprised a 140-amino acid protein with ATG (methionine) as the initiation codon, which is consistent with the molecular weight determined by SDS-polyacrylamide gel electrophoresis. The uvsY gene was oriented in the direction of the early genes and a sequence common to the middle promoter consensus was found in the 5'-upstream region.

摘要

我们已经确定了噬菌体T4的uvsY基因所在的一个1001碱基对区域的核苷酸序列。发现一个420个碱基对的开放阅读框编码uvsY基因产物。uvsY基因包含一个由140个氨基酸组成的蛋白质，起始密码子为ATG（甲硫氨酸），这与通过SDS-聚丙烯酰胺凝胶电泳测定的分子量一致。uvsY基因的方向是朝着早期基因的方向，并且在5'上游区域发现了一个与中间启动子共有序列相同的序列。