Owen J E, Schultz D W, Taylor A, Smith G R
J Mol Biol. 1983 Apr 5;165(2):229-48. doi: 10.1016/s0022-2836(83)80255-1.
The nucleotide sequence of the lysozyme (e) gene of bacteriophage T4 and approximately 130 additional nucleotides on each side has been determined. The 5'-end of the gene for internal protein III appears to be located about 70 base-pairs from the 3'-end of the lysozyme gene. Nucleotide sequence analysis of mutant e genes confirmed that three identified hotspots of frameshift mutations are runs of five A nucleotides in the wild-type gene. The endpoints of two deletions are direct repeats of eight base-pairs in the wild-type gene. Two frameshift mutations with high reversion frequencies are duplications of five or seven base-pairs. The cloning and nucleotide sequence determination of the lysozyme gene will facilitate further study of the molecular biology of T4 lysozyme.
噬菌体T4溶菌酶（e）基因的核苷酸序列以及两侧各约130个额外核苷酸已被确定。内部蛋白III基因的5'端似乎位于距溶菌酶基因3'端约70个碱基对处。突变e基因的核苷酸序列分析证实，三个已确定的移码突变热点是野生型基因中五个A核苷酸的序列。两个缺失的端点是野生型基因中八个碱基对的直接重复序列。两个具有高回复频率的移码突变是五个或七个碱基对的重复序列。溶菌酶基因的克隆和核苷酸序列测定将有助于进一步研究T4溶菌酶的分子生物学。
