爱泼斯坦-巴尔病毒（EBV）对人上皮细胞的感染。II. 上皮细胞中合成的EBV决定蛋白的生化特性

Infection of human epithelial cells by Epstein-Barr virus (EBV). II. Biochemical characterization of EBV-determined proteins synthesized in epithelial cells.

作者信息

Sinangil F, Volsky B, Volsky D J

出版信息

Virus Res. 1985 Oct;3(3):207-12. doi: 10.1016/0168-1702(85)90046-2.

Abstract

Primary cultures of epithelial cells were grown from tonsils of patients with diseases not related to EBV. The cells were implanted with EBV receptors and exposed to EBV of the transforming (B95-8, AG-876) and nontransforming (P3HR-1) strains. The EBV-infected and control cells were pulsed with [35S]methionine at 18-24 h after infection, and cell extracts were prepared for immunoprecipitation with anti-EBV sera and analysis by gel electrophoresis and autoradiography. About 20 EBV-determined proteins ranging from 22 to 185 kDa were detected in P3HR-1 virus-infected epithelial cells. Only a few polypeptides were detected in extracts of cells infected with AG-876 virus while no EBV-specific proteins were immunoprecipitated from extracts of B95-8 virus-infected cells. These results demonstrate that the system of EBV receptor-implanted normal human epithelial cells can be used for direct biochemical analysis of EBV infection in the epithelial tissue.

摘要

从与EBV无关疾病患者的扁桃体中培养出上皮细胞原代培养物。将这些细胞植入EBV受体，并暴露于转化型（B95-8、AG-876）和非转化型（P3HR-1）毒株的EBV中。在感染后18 - 24小时，用[35S]甲硫氨酸脉冲处理EBV感染的细胞和对照细胞，制备细胞提取物，用抗EBV血清进行免疫沉淀，然后通过凝胶电泳和放射自显影进行分析。在P3HR-1病毒感染的上皮细胞中检测到约20种由EBV决定的蛋白质，分子量在22至185 kDa之间。在AG-876病毒感染的细胞提取物中仅检测到少数几种多肽，而从B95-8病毒感染的细胞提取物中未免疫沉淀出EBV特异性蛋白质。这些结果表明，植入EBV受体的正常人上皮细胞系统可用于上皮组织中EBV感染的直接生化分析。