Yao Yanfeng, He Bin, Shao Zhiyong, Yang Wenhai, Liu Wu, Chen Xiabing, Ye Shengqiang, Chen Jie
Bing Du Xue Bao. 2016 Sep;32(5):590-6.
In 2016,routine influenza virus surveillance was conducted in the live poultry markets of Wuhan, Hubei Province. An H5N2 subtype avian influenza virus(AIV)was isolated from ducks in Wuhan. The entire genome of this virus isolate was sequenced,and molecular phylogenetic analysis performed. The results indicated that the HA gene belonged to clade 2.3.4.4and contained multiple basic amino acids at the cleavage site, which is characteristic of highly pathogenic AIV. Sequence alignment revealed that the isolate shared a high degree of homology with different H5 subtype AIVs isolated from waterfowl in southern China in recent years. This isolate was likely a natural reassortant from different subtype AIVs. This study provides epidemiological evidence of influenza evolution. Continuation of molecular epidemiology studies of H5 subtype influenza viruses in live poultry markets is important for understanding their role in the variation and evolution of highly pathogenic AIVs and their potential hazardous effects on human health. Furthermore, this information is important for strengthening comprehensive AIV surveillance and control measures.
2016年，在湖北省武汉市的活禽市场开展了常规流感病毒监测。从武汉的鸭子中分离出一株H5N2亚型禽流感病毒（AIV）。对该病毒分离株的全基因组进行了测序，并进行了分子系统发育分析。结果表明，该病毒的血凝素（HA）基因属于2.3.4.4分支，且在裂解位点含有多个碱性氨基酸，这是高致病性禽流感病毒的特征。序列比对显示，该分离株与近年来在中国南方水禽中分离出的不同H5亚型禽流感病毒具有高度同源性。该分离株可能是不同亚型禽流感病毒的自然重配体。本研究为流感病毒进化提供了流行病学证据。持续开展活禽市场H5亚型流感病毒的分子流行病学研究，对于了解其在高致病性禽流感病毒变异和进化中的作用以及对人类健康的潜在危害具有重要意义。此外，这些信息对于加强禽流感病毒的综合监测和防控措施也很重要。
