Kanazawa S, Herzlich B, Herbert V
Am J Gastroenterol. 1985 Dec;80(12):964-9.
The effect of human bile on the binding of free cobalamin (Cbl) and of intrinsic factor-Cbl (IF-Cbl) complexes to intestine receptors was examined in in vitro receptor extracts made from guinea pig small bowel epithelial cells. Cbl and IF-Cbl complex binding to the receptors was measured in the effluent from a Sephadex G-200 column. Intrinsic factor-Cbl binding to the receptors in the control samples was 4.32 +/- 0.07 ng/animal (mean +/- SEM); addition of bile, saturated with excess 57Co-Cbl enhanced binding to 5.26 +/- 0.16 ng/animal (p less than 0.001). These data suggest that bile enhances binding of IF-Cbl complex onto ileal receptors. Whether bile also serves as "releasing factor" by releasing Cbl from intrinsic factor in vivo remains to be determined.
在由豚鼠小肠上皮细胞制备的体外受体提取物中，研究了人胆汁对游离钴胺素（Cbl）以及内因子 - Cbl（IF - Cbl）复合物与肠道受体结合的影响。通过Sephadex G - 200柱流出物测量Cbl和IF - Cbl复合物与受体的结合。对照样品中IF - Cbl与受体的结合量为4.32±0.07 ng/动物（平均值±标准误）；添加用过量57Co - Cbl饱和的胆汁后，结合量增加至5.26±0.16 ng/动物（p<0.001）。这些数据表明胆汁增强了IF - Cbl复合物与回肠受体的结合。胆汁在体内是否还通过从内因子释放Cbl而充当“释放因子”，仍有待确定。
