Stopa E G, O'Brien R, Katz M
Gastroenterology. 1979 Feb;76(2):309-14.
Colchicine can induce the malabsorption of vitamin B12 and other nutrients. Previous investigations have suggested but not proved that this malabsorption was due to a lesion in the ileal mucosa. Employing the receptor assay of M. Katz and B. A. Cooper (J Clin Invest 54:733-739, 1974), the authors have observed a dose-related, reversible reduction in the quantity of intrinsic factor-vitamin B12 (IF-B12) receptor (from 5.78 ng to 1.3 ng of B12 binding) in the intestinal mucosa of guinea pigs fed 0.05-0.25 mg/100 g colchicine/day for 3 days. Malabsorption of vitamin B12 was also demonstrated in vivo in similarly treated animals. Increasing intestinal motility with cascara sagrada had no effect on the IF-B12 receptor. The quantity of IF-B12 receptor and the amount of vitamin B12 absorbed increased markedly to greater than normal levels during recovery from a 3-day course of colchicine. The total number of intestinal cells decreased after colchicine administration and increased during recovery; however, the fluctuations observed were not sufficient to explain the changes in the quantity of receptor. Histologic examination of the ileal mucosa showed a decrease in the population of villus cells after colchicine. The correlation between the changes in receptor quantity and in vivo B12-absorption prove that the IF-B12 receptor is a critical limiting factor in B12 absorption.
秋水仙碱可导致维生素B12和其他营养素的吸收不良。以往的研究表明但未证实这种吸收不良是由于回肠黏膜病变所致。作者采用M. Katz和B. A. Cooper的受体检测方法（《临床研究杂志》54:733 - 739, 1974），观察到给豚鼠每日喂食0.05 - 0.25mg/100g秋水仙碱，持续3天，其肠黏膜中内因子 - 维生素B12（IF - B12）受体数量呈剂量相关的可逆性减少（从5.78ng的B12结合量降至1.3ng）。在同样处理的动物体内也证实了维生素B12的吸收不良。用神圣大黄增加肠道蠕动对IF - B12受体无影响。在从3天的秋水仙碱疗程恢复期间，IF - B12受体数量和维生素B12吸收量显著增加至高于正常水平。秋水仙碱给药后肠细胞总数减少，恢复期间增加；然而，观察到的波动不足以解释受体数量的变化。回肠黏膜的组织学检查显示秋水仙碱处理后绒毛细胞数量减少。受体数量变化与体内B12吸收之间的相关性证明IF - B12受体是B12吸收的关键限制因素。
