Svenson M, Bendtzen K
Allergy. 1985 Oct;40(7):529-34. doi: 10.1111/j.1398-9995.1985.tb00262.x.
Cyclosporin (Cs) inhibits the elaboration of the lymphokine leukocyte migration inhibitory factor (LIF) from human blood mononuclear cells (MNC) stimulated with recall antigen. This inhibition was counteracted by 3 X 10(-5) M dibutyryl-cyclic GMP and 8-bromo-cyclic GMP and by the cyclic GMP-agonists, sodium nitroprusside (NaNPr), ascorbic acid (As A), sodium azide (NaN3) and carbacholine. Using 5 X 10(-5) M NaNPr, 1 X 10(-3) M NaN3, or 3 X 10(-3) M As A, 25-50-, 4-8- and 2-3-fold elevations of MNC and T-lymphocyte cyclic GMP-levels were obtained independently of the presence of Cs. NaNPr was the most potent of these three cyclic GMP-agonists in counteracting the effect of Cs. The results indicate that intracellular cyclic GMP is a major factor involved in the reversal of Cs-induced inhibition of LIF-production. None of the cyclic GMP-analogues or -agonists by themselves possessed Interleukin 1-like activity, measured by their ability to induce LIF-production by macrophage-depleted T-lymphocytes challenged by recall antigen.
环孢素（Cs）可抑制经回忆抗原刺激的人血单核细胞（MNC）产生淋巴因子白细胞迁移抑制因子（LIF）。3×10⁻⁵M的二丁酰环磷鸟苷和8-溴环磷鸟苷以及环磷鸟苷激动剂硝普钠（NaNPr）、抗坏血酸（As A）、叠氮化钠（NaN₃）和卡巴胆碱可抵消这种抑制作用。使用5×10⁻⁵M的NaNPr、1×10⁻³M的NaN₃或3×10⁻³M的As A，无论Cs是否存在，MNC和T淋巴细胞的环磷鸟苷水平均可分别独立升高25 - 50倍、4 - 8倍和2 - 3倍。NaNPr是这三种环磷鸟苷激动剂中抵消Cs作用最有效的。结果表明，细胞内环磷鸟苷是参与逆转Cs诱导的LIF产生抑制作用的主要因素。通过它们诱导经回忆抗原刺激的巨噬细胞耗竭的T淋巴细胞产生LIF的能力来衡量，这些环磷鸟苷类似物或激动剂自身均不具有白细胞介素1样活性。
