Gottlieb A A, Farmer J L, Matzura C T, Hester R B, Rosenberg J S
J Immunol. 1984 Jan;132(1):256-60.
Human leukocyte dialysates contain components capable of amplifying cutaneous delayed-type hypersensitivity (DTH) reactions. In the present study, two such amplifiers, both less than 3500 m.w., were partially purified from human leukocyte dialysates by gel filtration on Sephadex G-10 followed by high pressure reverse-phase liquid chromatography. These amplifiers of DTH were examined for their effects on production of the migration inhibitory lymphokines leukocyte migration inhibition factor (LIF) and macrophage migration inhibition factor (MIF). The amplifiers were found to increase LIF and MIF production by antigen- or alloantigen-stimulated human peripheral blood lymphocytes in a dose-dependent fashion. Further analysis demonstrated that although antigen-stimulated T4 and T8 cell subpopulations could produce LIF activity under the assay conditions employed, amplification of lymphokine production by modulator was only observed with the T4 subset.
人白细胞透析液含有能够增强皮肤迟发型超敏反应（DTH）的成分。在本研究中，通过在Sephadex G - 10上进行凝胶过滤，然后进行高压反相液相色谱，从人白细胞透析液中部分纯化出两种分子量小于3500的此类增强剂。检测了这些DTH增强剂对迁移抑制性淋巴因子白细胞迁移抑制因子（LIF）和巨噬细胞迁移抑制因子（MIF）产生的影响。发现这些增强剂以剂量依赖的方式增加抗原或同种异体抗原刺激的人外周血淋巴细胞产生LIF和MIF。进一步分析表明，虽然在所用的检测条件下，抗原刺激的T4和T8细胞亚群能够产生LIF活性，但仅在T4亚群中观察到调节剂对淋巴因子产生的增强作用。
