Department of Entomology and Agrilife Research, Texas A&M University, College Station, TX 77843, USA.
Viruses. 2018 Jul 12;10(7):367. doi: 10.3390/v10070367.
Several medically important flaviviruses that are transmitted by mosquitoes have been shown to bind to the C-type lectin fold that is present in either vertebrate or invertebrate proteins. While in some cases this interaction is part of a neutralizing anti-viral immune response, many reports have implicated this as critical for successful virus entry. Despite the establishment of mosquito C-type lectin domain containing proteins (CTLDcps) as known host factors in assisting the infectious process for flaviviruses, little is known about the structural characteristics of these proteins and their relationships to each other. In this report, we describe the manual annotation and structural characterization of 52 CTLDcps. Using existing RNAseq data, we establish that these genes can be subdivided into two classes: those highly conserved with expression primarily in development (embryo/early larvae) and those with no clear orthologs with expression primarily in late larvae/pupae or adults. The latter group contained all CTLDcps that are regulated by the Toll/Imd immune pathways, all known microbiome-regulating CTLDcps, and almost all CTLDcps that are implicated as flavivirus host factors in . Finally, we attempt to synthesize results from multiple conflicting gene expression profiling experiments in terms of how flavivirus infection changes steady-state levels of mRNA encoding CTLDcps.
几种通过蚊子传播的医学上重要的黄病毒已被证明能与脊椎动物或无脊椎动物蛋白中存在的 C 型凝集素折叠结合。虽然在某些情况下，这种相互作用是中和抗病毒免疫反应的一部分，但许多报告表明，这对于病毒的成功进入至关重要。尽管蚊子 C 型凝集素结构域包含蛋白 (CTLDcps) 已被确立为协助黄病毒感染过程的已知宿主因子，但人们对这些蛋白的结构特征及其相互关系知之甚少。在本报告中，我们描述了 52 个 CTLDcps 的手动注释和结构特征。利用现有的 RNAseq 数据，我们确定这些基因可以分为两类：一类高度保守，主要在发育过程中表达（胚胎/早期幼虫），另一类没有明显的直系同源物，主要在晚期幼虫/蛹或成虫中表达。后一组包含所有受 Toll/Imd 免疫途径调节的 CTLDcps、所有已知的微生物组调节 CTLDcps，以及几乎所有被认为是黄病毒宿主因子的 CTLDcps。最后，我们试图综合多个相互冲突的基因表达谱实验的结果，了解黄病毒感染如何改变 CTLDcps 编码 mRNA 的稳态水平。
