W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, United States.
Elife. 2017 Dec 5;6:e28844. doi: 10.7554/eLife.28844.
Transmission of dengue virus (DENV) requires successful completion of the infection cycle in the vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. While certain midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to DENV infection. We show that a () fungus, isolated from field-caught render the mosquito more permissive to DENV infection. This modulation is attributed to a profound down-regulation of digestive enzyme genes and trypsin activity, upon exposure to -secreted factors. In conclusion, we show for the first time that a natural mosquito gut-associated fungus can alter physiology in a way that facilitates pathogen infection.
登革热病毒（DENV）的传播需要在媒介中成功完成感染周期，这是在摄入感染性血液后从中肠组织开始的。虽然某些中肠相关细菌会影响病毒感染，但对于中肠相关真菌（真菌群落）及其成员如何影响对 DENV 感染的易感性知之甚少。我们发现，一种从野外捕获的（）真菌使蚊子更容易感染 DENV。这种调节归因于暴露于 - 分泌因子后消化酶基因和胰蛋白酶活性的深刻下调。总之，我们首次表明，一种天然的蚊子肠道相关真菌可以改变生理状态，从而促进病原体感染。
