CIRAD, UMR ASTRE, 97490, Sainte Clotilde, La Réunion, France.
ASTRE, University of Montpellier, CIRAD, INRAe, Montpellier, France.
Parasit Vectors. 2020 Aug 5;13(1):395. doi: 10.1186/s13071-020-04253-5.
Arthropod borne virus infections are the cause of severe emerging diseases. Among the diseases due to arboviruses, dengue (DEN) and Rift Valley fever (RVF) are in the top ten in the list of diseases responsible of severe human cases worldwide. Understanding the effects of viral infection on gene expression in competent vectors is a challenge for the development of early diagnostic tools and may enable researchers and policy makers to better anticipate outbreaks in the next future.
In this study, alterations in gene expression across the entire Aedes aegypti genome during infection with DENV and RVFV were investigated in vitro at two time points of infection, the early phase (24 h) and the late phase (6 days) of infection using the RNA sequencing approach RESULTS: A total of 10 upregulated genes that share a similar expression profile during infection with both viruses at early and late phases of infection were identified. Family B and D clip-domain serine proteases (CLIP) were clearly overrepresented as well as C-type lectins and transferrin.
Our data highlight the presence of 10 viral genes upregulated in Ae. aegypti during infection. They may also be targeted in the case of the development of broad-spectrum anti-viral diagnostic tools focusing the mosquito vectors rather than the mammalian hosts as they may predict the emergence of outbreaks.
节肢动物传播的病毒感染是严重新发疾病的原因。在由虫媒病毒引起的疾病中，登革热（DEN）和裂谷热（RVF）在全球范围内导致严重人类病例的疾病中排名前十。了解病毒感染对有能力的载体中基因表达的影响是开发早期诊断工具的一个挑战，这可能使研究人员和决策者能够更好地预测未来的疫情爆发。
在这项研究中，使用 RNA 测序方法在体外研究了 DENV 和 RVFV 感染后整个埃及伊蚊基因组在感染的两个时间点（感染的早期阶段（24 小时）和晚期阶段（6 天））的基因表达变化。
总共鉴定出了 10 个在两种病毒感染的早期和晚期阶段感染时具有相似表达谱的上调基因。B 族和 D 族剪接体丝氨酸蛋白酶（CLIP）以及 C 型凝集素和转铁蛋白明显过表达。
我们的数据强调了在埃及伊蚊感染期间上调的 10 个病毒基因的存在。在开发针对蚊媒而不是哺乳动物宿主的广谱抗病毒诊断工具的情况下，它们也可能成为目标，因为它们可能预测疫情的爆发。
