Kaplan N B, Grant M M, Brody J S
Am Rev Respir Dis. 1985 Dec;132(6):1307-12. doi: 10.1164/arrd.1985.132.6.1307.
We have previously shown that alveoli of neonatal rat lungs contain 2 morphologically distinct fibroblasts; 1 that contains lipid droplets and 1 that does not. We have named these lipid interstitial cells (LIC) and nonlipid interstitial cells (NLIC). In this study, using stereologic methods, we evaluated the distribution of LIC and NLIC in the alveoli of immature and mature rats, hamsters, and mice and quantitated cytoplasmic lipid in the LIC. We found that fibroblasts account for approximately 50% of resident alveolar wall cells in immature and 20% in mature rodent lungs. At each age LIC accounted for half of the fibroblasts. There were age and species differences in the amount of LIC lipid. The volume density of cytoplasmic lipid was higher in immature rats (0.285) than in mice (0.205) or hamsters (0.010) and was higher in mature mice (0.176) than in hamsters (0.051) or rats (0.019). Possible explanations for these differences are discussed. Bundles of characteristic cytoplasmic myofilaments established that the LIC was identical to the previously described contractile interstitial cell of the lung. These studies confirm the presence of 2 distinct fibroblasts in the alveoli of immature and mature rodents of several species.
我们之前已经表明，新生大鼠肺的肺泡含有两种形态上不同的成纤维细胞；一种含有脂滴，另一种不含。我们将这些分别命名为脂质间质细胞（LIC）和非脂质间质细胞（NLIC）。在本研究中，我们使用体视学方法评估了LIC和NLIC在未成熟和成熟大鼠、仓鼠及小鼠肺泡中的分布，并对LIC中的细胞质脂质进行了定量分析。我们发现，成纤维细胞在未成熟啮齿动物肺的驻留肺泡壁细胞中约占50%，在成熟啮齿动物肺中占20%。在每个年龄段，LIC占成纤维细胞的一半。LIC脂质的含量存在年龄和物种差异。未成熟大鼠（0.285）的细胞质脂质体积密度高于小鼠（0.205）或仓鼠（0.010），成熟小鼠（0.176）的高于仓鼠（0.051）或大鼠（0.019）。文中讨论了这些差异可能的解释。特征性细胞质肌丝束表明LIC与先前描述的肺收缩性间质细胞相同。这些研究证实了在几种未成熟和成熟啮齿动物的肺泡中存在两种不同的成纤维细胞。
