Brody J S, Kaplan N B
Am Rev Respir Dis. 1983 Jun;127(6):763-70. doi: 10.1164/arrd.1983.127.6.763.
In order to define the origin and fate of lipid-filled interstitial cells (LIC) in the neonatal rat lung, 4- and 11-day-old rats were injected with tritiated thymidine (3H-T) and killed 1 to 96 h later. Thymidine-labeled lung cells were identified in autoradiographs and analyzed ultrastructurally. Interstitial cells accounted for 61% of 3H-T-labeled cells at 4 days but only 26% at 11 days. The LIC had a different labeling index than did nonlipid interstitial fibroblasts (NLIC) at each age, and also had distinct morphologic characteristics. At no time point did labeled LIC lose their lipid and become NLIC or vice versa. These results suggest that at least 2 separate populations of interstitial fibroblasts exist in the neonatal rat lung, probably with distinct functions. This supports the concept of tissue fibroblast heterogeneity.
为了确定新生大鼠肺中脂质填充的间质细胞（LIC）的起源和命运，给4日龄和11日龄的大鼠注射氚标记的胸腺嘧啶核苷（³H-T），并在1至96小时后处死。在放射自显影片中鉴定出胸腺嘧啶核苷标记的肺细胞，并进行超微结构分析。间质细胞在4天时占³H-T标记细胞的61%，但在11天时仅占26%。在每个年龄段，LIC的标记指数与非脂质间质成纤维细胞（NLIC）不同，并且具有独特的形态学特征。在任何时间点，标记的LIC都不会失去其脂质并变成NLIC，反之亦然。这些结果表明，新生大鼠肺中至少存在2个独立的间质成纤维细胞群体，可能具有不同的功能。这支持了组织成纤维细胞异质性的概念。
