Ping Lifeng, Chen Fengmei, Cui Fengjie, Hu Wanjun, Sun Wenjing, Li Na, Yang Yan
1Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, Zhejiang University of Science and Technology, Hangzhou, 310023 People's Republic of China.
2School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 People's Republic of China.
3 Biotech. 2018 Jul;8(7):305. doi: 10.1007/s13205-018-1305-0. Epub 2018 Jul 5.
In this paper, the effects of erythorbic acid (EA) treatment with different concentrations on the quality of fruiting bodies stored at 4 °C for 27 days were studied by determining the changes in moisture content, weight loss, browning, electrolyte leakage, malondialdehyde (MDA), and nutritional compounds. The activities of polyphenoloxidase (PPO), cellulase and other antioxidant enzymes including superoxide dismutase (SOD), catalase, and peroxidase (POD) were also measured. Results showed that 0.1% EA-treated fruiting body maintained lower weight loss (< 6.0%, w/w), electrolyte leakage (< 45.8%), MDA (< 4.17 µmol kg), and higher moisture content (> 90.7%, w/w). Lower activities of PPO (< 72.64 × 10 U kg) and cellulase (< 189.86 × 10 U kg) in 0.1% EA-treated samples were observed compared with the other treatments. As a stereoisomer of ascorbic acid (AA), EA also could enhance SOD and POD activities of fruiting bodies. Our findings were the first time to evaluate the effect of EA on maintaining quality in fruiting bodies, and proved that low concentrations of EA (especially 0.1% EA, w/v) treatments were beneficial to preserve fruiting body with even higher efficiency than AA treatment. This study paved a foundation for the enhancement of quality retention of fruiting bodies.
本文通过测定水分含量、失重、褐变、电解质渗漏、丙二醛（MDA）和营养成分的变化，研究了不同浓度异抗坏血酸（EA）处理对在4℃下储存27天的子实体品质的影响。还测定了多酚氧化酶（PPO）、纤维素酶以及包括超氧化物歧化酶（SOD）、过氧化氢酶和过氧化物酶（POD）在内的其他抗氧化酶的活性。结果表明，0.1%EA处理的子实体保持较低的失重（<6.0%，w/w）、电解质渗漏（<45.8%）、MDA（<4.17µmol kg），以及较高的水分含量（>90.7%，w/w）。与其他处理相比，在0.1%EA处理的样品中观察到较低的PPO活性（<72.64×10 U kg）和纤维素酶活性（<189.86×10 U kg）。作为抗坏血酸（AA）的立体异构体，EA还可以提高子实体的SOD和POD活性。我们的研究结果首次评估了EA对子实体品质维持的影响，并证明低浓度的EA（尤其是0.1%EA，w/v）处理有利于保存子实体，其效率甚至高于AA处理。本研究为提高子实体的品质保留奠定了基础。
