De Lucca Leandro, da Costa Marques Márcio, Weinfeld Ilan
Clínica De Lucca & Marques Odontologia, Guarulhos, SP, Brazil.
Universidade de Santo Amaro - UNISA, São Paulo, SP, Brazil.
Heliyon. 2018 Jun 8;4(6):e00651. doi: 10.1016/j.heliyon.2018.e00651. eCollection 2018 Jun.
This study aimed to evaluate the bone formation process in experimental defects created on rabbit calvarial, in which one of the bone defects was covered by the impermeable membrane before suturing the skin flap, while the other was closed only by the cutaneous flap. The experimental holes were filled only by the blood clot.
Sixteen New Zealand female rabbits weighing between 3.5 and 4 kg were used. Two experimental bone defects were made in the rabbit calvarial. The holes were filled only with the blood clot and one of them was covered with an impermeable polypropylene membrane. A histological analysis was made at 21 and 42 days following the surgery. Histological evaluation consisted of the following: 1. inflammatory process; 2. Bone repair; 3. Bone remodeling; 4. Presence of osteoid matrix and mineralization, and 5. Formation of hematopoietic tissue. Each characteristic was analyzed semi quantitatively.
There was a statistical difference between the test and the control group at 21 days of healing in the following items: presence of cementation line (p = 0.012), presence of osteoid tissue (p = 0.012), and trabecular bone tissue development and mineralization (p = 0.012). A greater amount of lamellar bone tissue (mature) was also observed in the test group compared to the control group.
The semiquantitative analysis showed that at 21 days there was a superiority of the repair process in the test group; at 42 days there was no significant difference in bone formation between the two groups; and that the polypropylene membrane is feasible to be used in GBR.
The impermeable polypropylene barrier is feasible for use in the guided bone regeneration technique. It can be used only on the blood clot, without the need for grafting, and can be easily removed a few days after surgery. These results are unprecedented.
本研究旨在评估兔颅骨实验性缺损的骨形成过程，其中一个骨缺损在缝合皮瓣前用不可渗透膜覆盖，而另一个仅用皮瓣封闭。实验孔仅由血凝块填充。
使用16只体重在3.5至4千克之间的新西兰雌性兔。在兔颅骨上制造两个实验性骨缺损。孔仅用血凝块填充，其中一个用不可渗透的聚丙烯膜覆盖。术后21天和42天进行组织学分析。组织学评估包括以下方面：1. 炎症过程；2. 骨修复；3. 骨重塑；4. 类骨质基质和矿化的存在，以及5. 造血组织的形成。对每个特征进行半定量分析。
在愈合21天时，试验组和对照组在以下项目上存在统计学差异：胶结线的存在（p = 0.012）、类骨组织的存在（p = 0.012）以及小梁骨组织发育和矿化（p = 0.012）。与对照组相比，试验组还观察到更多的板层骨组织（成熟）。
半定量分析表明，在21天时试验组的修复过程更具优势；在42天时，两组之间的骨形成没有显著差异；并且聚丙烯膜可用于引导骨再生（GBR）。
不可渗透的聚丙烯屏障可用于引导骨再生技术。它仅可用于血凝块，无需植骨，并且在手术后几天可轻松取出。这些结果是前所未有的。
