Chang Ting-Kai, Chen Yuh-Kun
Master Program for Plant Medicine and Good Agricultural Practice, National Chung Hsing University, Taichung, 402, Taiwan.
Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan.
Arch Virol. 2018 Oct;163(10):2925-2928. doi: 10.1007/s00705-018-3940-5. Epub 2018 Jul 12.
Plants of the species Gomphocarpus physocarpus, commonly known as balloon plant or swan plant, exhibiting virus-like symptoms of mosaic, mottle and crinkling were observed and collected in the southwestern part of Taiwan in 2015. Electron microscopic examination showed the presence of virus-like flexuous-rod particles. Potyvirus pinwheel-shape and laminated inclusion bodies were observed in the preparations of ultrathin sections of diseased leaves. The complete genome sequence of the potyvirus of balloon plant was determined. It is 9998 nucleotides in length, excluding the 3'-terminal poly(A) tail. It contains two open reading frames encoding a polyprotein of 3196 amino acids and a PIPO protein of 89 amino acids. The polyprotein gene shares 52.8-68.4% nucleotide sequence identity and 40.2-75.8% amino acid sequence identity with other potyviruses tested. Molecular analysis indicates that the virus is most closely related to but distinct from keunjorong mosaic virus (KjMV). The virus causing mosaic, mottle and crinkling on Gomphocarpus plants (gomphocarpus mosaic virus, GoMV) likely belongs to a new species of the genus Potyvirus.
2015年，在台湾西南部观察并采集到了牛角瓜属植物（学名：Gomphocarpus physocarpus，俗称气球花或天鹅花），这些植株表现出类似病毒的花叶、斑驳和皱缩症状。电子显微镜检查显示存在类似病毒的弯曲杆状颗粒。在患病叶片超薄切片的制备物中观察到了马铃薯Y病毒属的风车状和层状内含体。测定了气球花马铃薯Y病毒的完整基因组序列。其长度为9998个核苷酸，不包括3'末端的聚腺苷酸尾巴。它包含两个开放阅读框，分别编码一个由3196个氨基酸组成的多聚蛋白和一个由89个氨基酸组成的PIPO蛋白。多聚蛋白基因与其他受试马铃薯Y病毒的核苷酸序列同一性为52.8 - 68.4%，氨基酸序列同一性为40.2 - 75.8%。分子分析表明，该病毒与克恩乔龙花叶病毒（KjMV）关系最为密切，但又有所不同。在牛角瓜属植物上引起花叶、斑驳和皱缩的病毒（牛角瓜花叶病毒，GoMV）可能属于马铃薯Y病毒属的一个新种。
