Xie Lixue, Gao Fangluan, Zheng Shan, Zhang Xiaoyan, Zhang Lijie, Li Tao
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, People's Republic of China.
Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
Arch Virol. 2019 Jul;164(7):1903-1906. doi: 10.1007/s00705-019-04251-8. Epub 2019 Apr 9.
A potyvirus (isolate PFV-FJ) infecting passion fruit in China was identified by small-RNA sequencing. The complete genome sequence of PFV-FJ was determined to be 9974 nucleotides, excluding the poly(A) tail. PFV-FJ shares 70-72% nucleotide and 69-74% amino acid sequence identity at the polyprotein level with seven reported potyviruses, but 89% nucleotide and 91% amino acid sequence identity with an unreported potyvirus, tentatively named "passionfruit Vietnam potyvirus" (PVNV-DakNong). This suggests that PFV-FJ and PVNV-DakNong should belong to the same potyvirus species and that PFV-FJ is a new member of the genus Potyvirus. This new potyvirus was tentatively named "passion fruit severe mottle-associated virus".
通过小RNA测序鉴定出一种在中国感染西番莲的马铃薯Y病毒(分离株PFV-FJ)。PFV-FJ的完整基因组序列经测定为9974个核苷酸,不包括多聚(A)尾。PFV-FJ在多聚蛋白水平上与七种已报道的马铃薯Y病毒具有70-72%的核苷酸和69-74%的氨基酸序列同一性,但与一种未报道的马铃薯Y病毒(暂命名为“越南西番莲马铃薯Y病毒”,PVNV-DakNong)具有89%的核苷酸和91%的氨基酸序列同一性。这表明PFV-FJ和PVNV-DakNong应属于同一马铃薯Y病毒种,且PFV-FJ是马铃薯Y病毒属的一个新成员。这种新的马铃薯Y病毒被暂命名为“西番莲严重斑驳相关病毒”。