Tsuka Yuji, Kunimatsu Ryo, Gunji Hidemi, Nakajima Kengo, Kimura Aya, Hiraki Tomoka, Nakatani Ayaka, Tanimoto Kotaro
Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, Hiroshima, Japan.
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
Lasers Med Sci. 2019 Feb;34(1):55-60. doi: 10.1007/s10103-018-2586-6. Epub 2018 Jul 12.
Low-level laser therapy has become one of the fastest growing fields of medicine in recent years. Many in vivo and in vitro studies have shown that laser irradiation activates a range of cellular processes in a variety of cell types and can promote tissue repair. However, few in vitro experiments have evaluated the effects of laser irradiation on cells in real time. The purpose of this study was to examine the effects of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on the migration of cultured human osteoblasts. A dedicated 96-well plate was used, and confluent cultures of the human osteoblast-like cell line, Saos-2, were injured with a wound maker. The wounded cells were then exposed to the Nd:YAG laser (wavelength of 1064 nm) for 60 s at 0.3 W (10 pps, 30 mJ). The total energy density was about 10.34 J/cm. Images of the wounds were automatically acquired inside the CO incubator by the IncuCyte ZOOM™ software. In addition, after laser irradiation, the production of adenosine triphosphate (ATP) was measured using the CellTiter-Glo™ Luminescent Cell Viability Assay. Migration of cells from the border of the original scratch zone was accelerated by laser irradiation. In addition, compared with the control group, significant enhancement of ATP production was observed in the irradiated group. The present study showed that Nd:YAG laser irradiation (wavelength of 1064 nm, 0.3 W, 10 pps, 30 mJ, 10.34 J/cm, irradiation time 60 s) may contribute to the regeneration of bone tissues owing to enhanced osteoblast cell migration.
近年来，低强度激光疗法已成为医学领域中发展最快的领域之一。许多体内和体外研究表明，激光照射可激活多种细胞类型中的一系列细胞过程，并能促进组织修复。然而，很少有体外实验实时评估激光照射对细胞的影响。本研究的目的是探讨掺钕钇铝石榴石（Nd:YAG）激光照射对培养的人成骨细胞迁移的影响。使用了专用的96孔板，用人成骨样细胞系Saos-2的汇合培养物用伤口制作器造成损伤。然后将受伤的细胞暴露于Nd:YAG激光（波长1064nm）下，以0.3W（10pps，30mJ）照射60秒。总能量密度约为10.34J/cm²。通过IncuCyte ZOOM™软件在CO₂培养箱内自动获取伤口图像。此外，激光照射后，使用CellTiter-Glo™发光细胞活力测定法测量三磷酸腺苷（ATP）的产生。激光照射加速了细胞从原始划痕区边界的迁移。此外，与对照组相比，照射组的ATP产生显著增强。本研究表明，Nd:YAG激光照射（波长1064nm，0.3W，10pps，30mJ，10.34J/cm²，照射时间60秒）可能由于成骨细胞迁移增强而有助于骨组织的再生。
