Xue Guoping, Wang Shirui, Liu Qingmei, Zhang Kuanshou, Xin Pengfei
Department of Stomatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, 030032, Shanxi, China.
Shanxi Medical University, School and Hospital of Stomatology, Taiyuan, 030000, Shanxi, China.
Heliyon. 2023 Jan 4;9(1):e12800. doi: 10.1016/j.heliyon.2023.e12800. eCollection 2023 Jan.
The aim of this study is to evaluate whether concentrated growth factor (CGF) and photobiomodulation (PBMT) can show synergistic effect on bone healing process.
In vivo osteogenesis studies were performed in a rabbit critical-sized calvarial defect model. Four 8 mm critical-sized bone defects were created on each rabbit calvarium, and these 4 defects were randomly divided into 4 groups: 1-control (defect filled with autologous blood clot); 2-CGF (defect filled with CGF); 3-LLLT (defect filled with autologous blood clot and received Nd:YAG low-level laser irradiation); 4-CGF + LLLT (defect filled with CGF and received LLLT). 15 Japanese big-ear white rabbits were operated on using the same procedure in this study. Then, 5 rabbits were selected randomly and sacrificed at 4th, 6th and 8th week postoperatively and respectively. The calvariums were harvested and scanned by micro-CT. The volumes of new bone formation of these defects were calculated by analyzing the micro-CT image. Data were analyzed as mean values of each group, comparisons were made for statistical analysis with the group and among the 4 groups using analysis of variance (ANOVA, P < 0.05).
At the 4th, 6th and 8th weeks, compared with the control group, the volume of new bone formed in each experimental group was significantly increased. Both CGF and LLLT can accelerate bone healing, but the effect of LLLT is better than that of CGF, and the difference between the two is statistically significant (P < 0.01). There was no statistically significant difference in the osteogenic effect between the combined application of CGF + LLLT and the application of CGF alone. And the osteogenic effect of the former two groups was weaker than that obtained by laser irradiation alone.
Both CGF and LLLT can promote osteogenesis effectively, but the combination of the two did not show a synergistic effect. The pro-osteogenic effect of Nd:YAG low-level laser irradiation is superior to that of CGF, and also superior to the combined effect of the two.
本研究旨在评估浓缩生长因子（CGF）和光生物调节（PBMT）对骨愈合过程是否具有协同作用。
在兔临界大小颅骨缺损模型中进行体内成骨研究。在每只兔颅骨上制作4个8毫米的临界大小骨缺损，并将这4个缺损随机分为4组：1-对照组（缺损填充自体血凝块）；2-CGF组（缺损填充CGF）；3-低强度激光治疗组（缺损填充自体血凝块并接受Nd:YAG低强度激光照射）；4-CGF + 低强度激光治疗组（缺损填充CGF并接受低强度激光照射）。本研究中15只日本大耳白兔采用相同手术程序进行手术。然后，随机选择5只兔子，分别在术后第4、6和8周处死。采集颅骨并进行微型计算机断层扫描（micro-CT）。通过分析micro-CT图像计算这些缺损处新骨形成的体积。数据以每组平均值进行分析，使用方差分析（ANOVA，P < 0.05）对组内和4组之间进行比较以进行统计分析。
在第4、6和8周时，与对照组相比，各实验组新形成的骨体积均显著增加。CGF和低强度激光治疗均能加速骨愈合，但低强度激光治疗的效果优于CGF，两者差异具有统计学意义（P < 0.01）。CGF + 低强度激光治疗联合应用与单独应用CGF之间的成骨效果无统计学显著差异。且前两组的成骨效果弱于单独激光照射所获得的效果。
CGF和低强度激光治疗均能有效促进成骨，但两者联合应用未显示出协同作用。Nd:YAG低强度激光照射的促骨生成作用优于CGF，也优于两者联合应用的效果。
