Tsuka Yuji, Kunimatsu Ryo, Gunji Hidemi, Sakata Shuzo, Nakatani Ayaka, Oshima Sho, Rikitake Kodai, Aisyah Purtranti Nurul, Kado Isamu, Ito Shota, Tanimoto Kotaro
Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima, Japan.
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical & Health Sciences,, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
J Lasers Med Sci. 2023 Mar 30;14:e9. doi: 10.34172/jlms.2023.09. eCollection 2023.
A variety of laser treatments have been applied in numerous medical fields. In dentistry, laser treatments are used for caries, root canals, and periodontal disease, as well as surgical resection. Numerous reports have recently been published on the use of lasers for bone regeneration. If laser irradiation is found to promote the activation of bone metabolism, it might also be effective for periodontal treatment, peri-implantitis, and bone regeneration. Therefore, the present study aimed to elucidate the mechanisms underlying the effects of erbium-doped yttrium aluminum garnet (Er: YAG) laser irradiation on the bone using osteoblast-like cells. Osteoblast-like Saos 2 cells (5.0×10 cells) were seeded in 24-well plates. 24 hours after being seeded, the cells were subjected to 0.3 W, 0.6 W, and 2.0 W Er: YAG laser irradiation and then allowed to recover for 48 hours. The expression levels of bone metabolism-related factors alkaline phosphatase (ALP), bone sialoprotein (BSP), and osteoprotegerin (OPG) were then evaluated using reverse transcription-quantitative polymerase chain reaction and western blot analyses. Saos 2 cells subjected to Er: YAG laser irradiation at 0.3 W, 0.6 W, and 2.0 W showed normal growth. When the Er: YAG laser irradiation and control groups were compared after 48 hours, increases were observed in ALP, BSP, and OPG gene and protein expression in the 2.0 W group. Similar results were obtained in the western blot analysis. These findings suggest that the Er: YAG laser irradiation of osteoblast-like cells is effective for activating bone metabolism factors.
多种激光治疗已应用于众多医学领域。在牙科领域，激光治疗用于龋齿、根管治疗、牙周疾病以及手术切除。最近有大量关于使用激光促进骨再生的报道。如果发现激光照射能促进骨代谢的激活，那么它可能对牙周治疗、种植体周围炎和骨再生也有效。因此，本研究旨在使用成骨样细胞阐明掺铒钇铝石榴石（Er:YAG）激光照射对骨作用的潜在机制。将成骨样Saos 2细胞（5.0×10个细胞）接种于24孔板中。接种24小时后，对细胞进行0.3W、0.6W和2.0W的Er:YAG激光照射，然后使其恢复48小时。随后使用逆转录定量聚合酶链反应和蛋白质印迹分析评估骨代谢相关因子碱性磷酸酶（ALP）、骨涎蛋白（BSP）和骨保护素（OPG）的表达水平。接受0.3W、0.6W和2.0W Er:YAG激光照射的Saos 2细胞生长正常。48小时后比较Er:YAG激光照射组和对照组时，发现2.0W组的ALP、BSP和OPG基因及蛋白表达增加。蛋白质印迹分析也得到了类似结果。这些发现表明，对成骨样细胞进行Er:YAG激光照射可有效激活骨代谢因子。
