Huang Jun, Yin Lei, Dong Lin, Quan Hongfeng, Chen Rong, Hua Shiyao, Ma Jiahua, Guo Dongyan, Fu Xueyan
School of Pharmacy, Ningxia Medical University, Yinchuan, China.
Shizuishan Maternal and Child Health Care Family Planning Service Center, Shizuishan, China.
Biomed Chromatogr. 2018 Nov;32(11):e4343. doi: 10.1002/bmc.4343. Epub 2018 Aug 23.
Radix Astragali (RA) is one of the most widely used Chinese herbs prescribed in many Chinese formulas to reinforce 'Qi' and treat vital energy deficiency. This study combined fingerprinting with quantitative analysis multi-components by a single marker (QAMS) to improve the quality control standard for RA on the basis of existing quality control methods of traditional Chinese medicinal materials. UPLC-ESI-TOF-MS technique was used to evaluate the quality of RA by fingerprinting and QAMS. Using the anti-inflammatory, anti-oxidation and anti-anoxic activities to screen characteristic components of RA, the calycosin-7-O-β-d-glucoside (CG), ononin, astragaloside IV, astragaloside II, calycosin and astrageloside I significantly inhibited ear edema in mice, the calycosin and CG had good antioxidant activity and the astragaloside I had a significant anti-hypoxia activity. Astragaloside I, astragaloside II, astragaloside IV, ononin, calycosin and CG had significant pharmacological actions. These components were comprehensively used as the indicative components for the quality control of RA. Astragaloside I was used as the internal standard of the relative correction factors of CG (13.45), ononin (0.51), calycosin (12.08), astragaloside IV (0.73) and astragaloside II (0.81). Astragaloside I and CG were used as internal standards of the relative correction factors of the flavonoids and saponins of ononin (1.11), calycosin (0.04), astragaloside IV (0.73) and astragaloside II (0.81). The study combined fingerprinting with QAMS to improve the quality control standard for RA.
黄芪是许多中药配方中最常用的中药材之一，用于补气和治疗气虚。本研究将指纹图谱与一测多评法（QAMS）相结合，在现有中药材质量控制方法的基础上，提高黄芪的质量控制标准。采用超高效液相色谱-电喷雾电离-飞行时间质谱（UPLC-ESI-TOF-MS）技术，通过指纹图谱和QAMS对黄芪质量进行评价。利用抗炎、抗氧化和抗缺氧活性筛选黄芪的特征成分，毛蕊异黄酮葡萄糖苷（CG）、芒柄花苷、黄芪甲苷、黄芪乙苷、毛蕊异黄酮和黄芪苷I对小鼠耳肿胀有显著抑制作用，毛蕊异黄酮和CG具有良好的抗氧化活性，黄芪苷I具有显著的抗缺氧活性。黄芪苷I、黄芪苷II、黄芪甲苷、芒柄花苷、毛蕊异黄酮和CG具有显著的药理作用。这些成分综合用作黄芪质量控制的指示性成分。黄芪苷I用作CG（13.45）、芒柄花苷（0.51）、毛蕊异黄酮（12.08）、黄芪甲苷（0.73）和黄芪乙苷（0.81）相对校正因子的内标。黄芪苷I和CG用作芒柄花苷（1.11）、毛蕊异黄酮（0.04）、黄芪甲苷（0.73）和黄芪乙苷（0.81）黄酮类和皂苷类相对校正因子的内标。本研究将指纹图谱与QAMS相结合，提高了黄芪的质量控制标准。
