Liu Menghua, Li Panlin, Zeng Xuan, Wu Huanxian, Su Weiwei, He Jingyu
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
State Key Laboratory of Organ Failure Research, Guangdong Provincial Institute of Nephrology, Southern Medical University, Guangzhou 510515, China.
Int J Mol Sci. 2015 Mar 5;16(3):5047-71. doi: 10.3390/ijms16035047.
Radix Astragali (RA) is one of the commonly-used traditional Chinese medicines (TCMs) with an immunomodulatory effect confirmed in the clinic. In order to better understand the material basis for the therapeutic effects, this study was to investigate the absorbed components and their pharmacokinetic profile after oral administration of RA on cyclophosphamide-induced immunosuppression in Balb/c mice. As a result, 51 compounds in RA extract and 31 prototype compounds with nine metabolites were detected in mice plasma by the ultra-fast liquid chromatography (UFLC)-DAD-Q-TOF-MS/MS method. The pharmacokinetic parameters of five main constituents, including calycosin-7-O-glucoside, ononin, calycosin, formononetin and astragaloside IV, were obtained using HPLC-MS/MS. These results offered useful information for research on the pharmacological mechanism of RA and for its further development.
黄芪是临床已证实具有免疫调节作用的常用中药之一。为了更好地理解其治疗作用的物质基础，本研究旨在考察黄芪口服给药后在环磷酰胺诱导免疫抑制的Balb/c小鼠体内的吸收成分及其药代动力学特征。结果，采用超快速液相色谱（UFLC）-DAD-Q-TOF-MS/MS法在小鼠血浆中检测到黄芪提取物中的51种化合物以及31种原型化合物和9种代谢产物。使用HPLC-MS/MS获得了包括毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素和黄芪甲苷IV在内的5种主要成分的药代动力学参数。这些结果为黄芪药理机制的研究及其进一步开发提供了有用信息。
