Ma Qianzhang, Ding Yuanquan, Wu Zhenqi, Li Yan
Department of Gastroenterology, Shengjing Hospital affiliated to China Medical University, Shenyang, 110004, China.
J BUON. 2018 May-Jun;23(3):587-591.
Emodin is an important constituent of Rheum emodi, an important medicinal herb. Emodin has been reported to exhibit significant pharmacological potential. Several activities such as anticancer activity have been attributed to emodin. However, the anticancer effects of emodin on colon cancer cells have not been fully studied. Therefore, the present study was designed to investigate the anticancer activity of emodin against the CACO-2 colon carcinoma cells.
The anti-proliferative activity of emodin was assessed by MTT assay. Apoptosis, and cell cycle analysis were carried out by flow cytometry using different fluorescent probes. Expression of proteins was examined by western blotting.
The results indicated that emodin reduced the viability of CACO-2 colon cancer cells. The observed IC50 for emodin was 30 μM at 24 hrs of incubation. Furthermore, the anticancer effects of emodin were found to be due to induction of apoptosis. Mitochondrial membrane potential (MMP) determination and Bax/Bcl-2 ratio indicated that emodin-induced apoptosis followed the mitochondrial pathway. Emodin could also trigger cell cycle arrest in CACO-2 colon carcinoma cells in a dose-dependent manner. Evaluation of the effect of emodin in PI3/AKT signalling pathway revealed that emodin could inhibit this signalling cascade indicating the potential of emodin as anticancer drug for the treatment of colon cancer.
Emodin exhibited potent anticancer effects in CACO-2 human colon carcinoma cells by inducing apoptosis, cell cycle arrest and inhibition of PI3K/AKT signalling pathway.
大黄素是重要药用植物掌叶大黄的一种重要成分。据报道，大黄素具有显著的药理潜力。大黄素具有多种活性，如抗癌活性。然而，大黄素对结肠癌细胞的抗癌作用尚未得到充分研究。因此，本研究旨在探讨大黄素对Caco-2结肠癌细胞的抗癌活性。
采用MTT法评估大黄素的抗增殖活性。使用不同荧光探针通过流式细胞术进行凋亡和细胞周期分析。通过蛋白质印迹法检测蛋白质表达。
结果表明，大黄素降低了Caco-2结肠癌细胞的活力。在孵育24小时时，观察到大黄素的IC50为30μM。此外，发现大黄素的抗癌作用是由于诱导凋亡。线粒体膜电位（MMP）测定和Bax/Bcl-2比值表明，大黄素诱导的凋亡遵循线粒体途径。大黄素还可使Caco-2结肠癌细胞以剂量依赖性方式发生细胞周期阻滞。对大黄素在PI3/AKT信号通路中的作用评估显示，大黄素可抑制该信号级联反应，表明大黄素作为治疗结肠癌的抗癌药物具有潜力。
大黄素通过诱导凋亡、细胞周期阻滞和抑制PI3K/AKT信号通路，在Caco-2人结肠癌细胞中表现出强大的抗癌作用。
