Zhou Tao, Liu Svetlana, Yang Li, Ju Yingchao, Li Chunxiao
Breast Disease Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050011, P.R. China.
J BUON. 2018 May-Jun;23(3):713-719.
To investigate the expression of ANXA3 in breast cancer tissues and normal breast tissues and its relationship with the clinicopathological features.
The expression levels of ANXA3 mRNA and protein were determined by fluorescent quantitative RT-PCR and Western blot, respectively, in 81 samples from breast cancer tissues and normal breast tissues. Flow cytometry, used to detect the cell cycle, further analyzed the differences in the cell proliferation indexes between cancer and the normal tissues. In addition, the correlation between the gene and protein expression levels of ANXA3 and the cell proliferation indexes in breast cancer tissues was calculated.
The expression levels of ANXA3 mRNA and protein were significantly higher in breast cancer tissues than in control samples. Furthermore, their expression patterns were significantly different in patients with different axillary lymph node metastasis states, with or without vascular tumor thrombus, and with different molecular classifications. The expression of ANXA3 mRNA and protein in patients with triple-negative breast cancer was significantly higher than in Luminal A and B types. However, no significant differences were detected in the expression levels in other subtypes. Compared to normal tissues, the cell proliferation indexes of breast cancer tissues were significantly higher, and presented significant positive correlations with the ANXA3 gene and protein expression levels.
The expression of ANXA3 might play a crucial role in promoting the occurrence, development, and metastasis of breast cancer. It could serve as an important marker involved in the invasion and metastasis states, and the prognosis of breast cancer.
探讨膜联蛋白A3（ANXA3）在乳腺癌组织和正常乳腺组织中的表达情况及其与临床病理特征的关系。
分别采用荧光定量逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测81例乳腺癌组织和正常乳腺组织样本中ANXA3 mRNA和蛋白的表达水平。运用流式细胞术检测细胞周期，进一步分析癌组织与正常组织之间细胞增殖指数的差异。此外，计算乳腺癌组织中ANXA3基因和蛋白表达水平与细胞增殖指数之间的相关性。
乳腺癌组织中ANXA3 mRNA和蛋白的表达水平显著高于对照样本。此外，在不同腋窝淋巴结转移状态、有无血管肿瘤血栓以及不同分子分型的患者中，其表达模式存在显著差异。三阴性乳腺癌患者中ANXA3 mRNA和蛋白的表达显著高于Luminal A型和B型。然而，在其他亚型中未检测到表达水平的显著差异。与正常组织相比，乳腺癌组织的细胞增殖指数显著更高，且与ANXA3基因和蛋白表达水平呈显著正相关。
ANXA3的表达可能在促进乳腺癌的发生、发展和转移中起关键作用。它可作为参与乳腺癌侵袭和转移状态以及预后的重要标志物。
