Zhou Tao, Li Yong, Yang Li, Tang Tiantian, Zhang Lina, Shi Jiajie
Department of Breast Cancer Center, The Fourth Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Department of General Surgery, The Fourth Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Biomed Res Int. 2017;2017:2603685. doi: 10.1155/2017/2603685. Epub 2017 Apr 13.
To validate the correlation between ANXA3 expression and prognosis in breast cancer, a retrospective study encompassing 309 breast cancer patients was performed. The expression of ANXA3 was determined by the immunohistochemical examination of tissue sections by the Max Vision™ method. The ANXA3 levels in the patient samples were validated for the prognosis based on age, menopause status, tumor size, tumor node, metastasis stage, the number of lymphatic metastases, oncology grade, and molecular subtyping. An elevated expression of ANXA3 was detected in breast cancer samples, compared to adjacent tissue samples, and significant correlation depending on the number of lymphatic metastases ( = 0.001) and histological grade ( = 0.004) was observed. The number of lymphatic metastases and ANXA3 expression were identified as independent risk factors affecting the disease-free survival and overall survival. Significantly ( < 0.002) higher level of ANXA3 was detected in triple-negative breast cancer compared to other subtypes. There was no significant ( > 0.05) change in the expression of ANXA3 with respect to age, menopausal status, tumor size, and clinical stage. The findings implicate the expression of ANXA3 with the natural progression of breast cancer and associate it with increased lymphatic metastasis. The study validates the use of ANXA3 as a potential prognosis biomarker for breast cancer.
为验证膜联蛋白A3（ANXA3）表达与乳腺癌预后之间的相关性，开展了一项纳入309例乳腺癌患者的回顾性研究。采用Max Vision™法通过组织切片免疫组化检查确定ANXA3的表达。根据年龄、绝经状态、肿瘤大小、肿瘤淋巴结、转移分期、淋巴转移数量、肿瘤学分级和分子亚型对患者样本中的ANXA3水平进行预后验证。与相邻组织样本相比，在乳腺癌样本中检测到ANXA3表达升高，并且观察到其与淋巴转移数量（P = 0.001）和组织学分级（P = 0.004）存在显著相关性。淋巴转移数量和ANXA3表达被确定为影响无病生存期和总生存期的独立危险因素。与其他亚型相比，三阴乳腺癌中检测到的ANXA3水平显著更高（P < 0.002）。ANXA3的表达在年龄、绝经状态、肿瘤大小和临床分期方面无显著变化（P > 0.05）。这些发现表明ANXA3的表达与乳腺癌的自然进展相关，并与淋巴转移增加有关。该研究验证了ANXA3作为乳腺癌潜在预后生物标志物的应用。
