Department of Chemistry and Nano Science , Ewha Womans University , Seoul 120-750 , Korea.
Department of Energy Systems Research , Ajou University , Suwon, Gyeonggi-do 443-749 , Korea.
Anal Chem. 2018 Aug 7;90(15):9510-9514. doi: 10.1021/acs.analchem.8b02195. Epub 2018 Jul 24.
The ratiometric fluorescent probe B6S, which contains pyrene as a fluorophore and imidazoline-2-thione as a reactive site, was developed for detection of hypochlorite (OCl). B6S displays a high specificity toward OCl in contrast to other reactive oxygen species and reactive nitrogen species. The probe has a low detection limit and operates under biological conditions. Moreover, the low cytotoxicity of B6S enables it to be utilized effectively for OCl imaging in living cells and tissues by using two-photon microscopy. The findings indicate that B6S has the capability of serving as a probe to explore the biological functions of OCl in living systems.
比率荧光探针 B6S 以芘为荧光团，以咪唑啉-2-硫酮为反应位点，用于检测次氯酸根（OCl）。与其他活性氧和活性氮物质相比，B6S 对 OCl 具有高度特异性。该探针具有较低的检测限，并且在生物条件下运行。此外，B6S 的低细胞毒性使其能够通过双光子显微镜有效地用于活细胞和组织中的 OCl 成像。研究结果表明，B6S 有能力作为一种探针，用于研究活系统中 OCl 的生物学功能。
