Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
Anal Chim Acta. 2013 May 2;775:100-5. doi: 10.1016/j.aca.2013.03.016. Epub 2013 Mar 15.
A fluorescence ratiometric sensor for OCl(-) has been developed based on a novel dual fluorophore response approach. The sensor molecule contains a coumarin fluorophore and a rhodamine fluorophore, and the two fluorophores are directly linked to an OCl(-) recognition group. The structure of the sensor was characterized by ESI-MS, NMR, and X-ray crystallographic analysis. Upon treatment with OCl(-), both fluorophores in the sensor responded simultaneously at two separate optical windows, with large enhancement of the fluorescence ratio (I578/I501) from 0.01 to 39.55. The fluorescence ratios for the sensor showed a good linearity with the concentration of OCl(-) in the range of 0.2-40 μM and the detection limits is 0.024 μM (SN(-1)=3). Investigation of reaction products indicated that the sensor reaction with OCl(-) produced two new fluorescent molecules, which were responsible for the fluorescence changes in two optical windows. In addition, the sensor showed high selectivity to OCl(-) over other reactive oxygen species, reactive nitrogen species, cations, and anions. The sensor has also been successfully applied to detection of OCl(-) in natural water samples with satisfactory recovery.
一种基于新型双重荧光响应策略的用于检测次氯酸根（OCl(-)）的荧光比率传感器已经被开发出来。该传感器分子包含香豆素荧光团和罗丹明荧光团，这两个荧光团直接连接到一个 OCl(-)识别基团上。传感器的结构通过 ESI-MS、NMR 和 X 射线晶体学分析进行了表征。在与 OCl(-) 处理后，传感器中的两个荧光团在两个单独的光学窗口中同时响应，荧光比率（I578/I501）从 0.01 增强到 39.55。传感器的荧光比率与 OCl(-) 的浓度在 0.2-40 μM 的范围内呈现出良好的线性关系，检测限为 0.024 μM（SN(-1)=3）。对反应产物的研究表明，传感器与 OCl(-) 的反应生成了两个新的荧光分子，这两个分子负责两个光学窗口中的荧光变化。此外，该传感器对 OCl(-) 具有高选择性，优于其他活性氧物质、活性氮物质、阳离子和阴离子。该传感器还成功地应用于天然水样中 OCl(-)的检测，具有令人满意的回收率。
